Phospho-Shp1 (Tyr536) (2A7) rabbit mAb

Catalog
2351
Select Your Size Price
200 uL (0.5 mg/mL)(2351) $475.00
20 uL (0.5 mg/mL)(2351S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description Src homology-2 domain containing phosphatase-1 (Shp1) typically functions as a negative regulator for signal transduction, interacting with Zap70, CD5, CD3ε, and IL-2R in T cells. Shp1 suppresses cellular signaling by dephosphorylating target proteins. Shp1 is expressed predominantly in hematopoietic cells, where it can plays a role in malignant transformation into cancer cells. Methylation of the Shp1 promoter has been shown to silence Shp1 expression and promote the development of cancer. Shp1 negatively regulates PDGF and insulin tyrosine receptor pathways, and negatively regulates Lck in the TCR signaling pathway to limit TCR responsiveness. Shp1 activity is prevented by TAOK3 in T cells to allow for T cell activation and differentiation.
Product Details
More Information
Applications Flow Cytometry
Clone Shp1Y536-2A7
Format Unconjugated
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr536 of human phospho Shp1
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Tyrosine-protein phosphatase non-receptor type 6, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, PTP-1C, SH-PTP1, PTPN6, HCP, PTP1C
Uniprot ID P29350
References Oka T, Ouchida M, Koyama M, et al. (2002) Cancer Research. 62:6390-6394.
Geraldes P, Hiraoka-Yamamoto J, Matsumoto M, Clermont A, Leitges M, Marette A, Aiello LP, Kern TS, and King GL. (2009) Nature Medicine. 15:1298-1306.
Ormonde JVS, Li Z, Stegen C, and Madrenas J. (2018) Journal of Immunology 201:3431-3442.
Images
Cell Culture Products
Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-Shp1 (Tyr536) antibody Shp1Y536-2A7 at 0.1 µg/mL. Cat. #2351.