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Phospho-Histone H3 (Ser28) (D6) rabbit mAb
20 μL is enough antibody for at least 20 Western blots.
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|Description||Histone H3 is one of the five main histone proteins involved in chromatin structure modification in eukaryotic cells. Histone proteins are highly post-translationally modified, including acetylation, phosphorylation, methylation and ubiquitination. Phospho histone H3 is the most extensively modified of the five histones. Phospho histone H3 is primarily acetylated at Lys9, 14, 18 and 23. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state.|
|Applications||Flow Cytometry, WB|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Ser28 of human phospho Histone H3|
|Validated Reactivity||Human, Mouse|
|Recommended Usage||1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|References||1. Rosenfeld JA, et al. (2009) BMC Genomics. 10: 143.
2. Lachner M et al. (2001) Nature. 410: 116–20.
3. Hansen JC, et al. (1998) Biochemistry. 37: 17637-41.
4. Strahl BD and Allis, CD (2000) Nature. 403: 41-45.
5. Cheung P, et al. (2000) Cell. 103: 263-271.
6. Bernstein BE, and Schreiber SL (2002) Chem. Biol. 9, 1167-1173.
7. Thorne AW, et al. (1990) Eur. J. Biochem. 193: 701-713.
8. Hendzel MJ, et al. (1997) Chromosoma. 106: 348-360.
9. Goto H, et al. (1999) J. Biol. Chem. 274: 25543-25549.
10. Preuss U, et al. (2003) Nucleic Acids Res. 31: 878-885.
Flow cytometric analysis of Hela secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green) using 0.05 µg/mL Phospho-Histone H3 (Ser28) antibody HisH3S28-D6. Cat. #2046.
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or untreated (red) or treated with UV and PMA (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 at 0.1µg/mL. Cat #2046.
Western blot analysis of Hela cell extract untreated or synchronized in metaphase by treatment with 0.4 ug/mL of nocodazole for 6 hr then metaphase cells were isolated by mitotic shake-off using Phospho-Histone H3 (Ser28) antibody at 1 ng/mL HisH3S28-D6. Cat. #2046.
Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 (Abwiz Cat. #2046) at 0.05ug/mL or Company C antibody at 0.2ug/mL (manufacturer's recommended concentration).
Western blot analysis of HeLa cells untreated or treated with nocodazole (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 (Abwiz Cat. #2046) at 0.005ug/mL or Company C antibody at 0.07ug/mL (manufacturer's recommended concentration) developed using the same exposure.
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