Phospho-Histone H3 (Ser10) (4B6) rabbit mAb

Catalog
2061
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200 uL (0.5 mg/mL)(2061) $475.00
20 uL (0.5 mg/mL)(2061S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description Histones are highly conserved proteins that serve the core of nucleosomes, which serve to organize chromatin fiber for DNA packing. Histone H3 phosphorylation plays a major role in both transcriptional activation, which requires unpacking of the chromatin structure, and in chromosome packing during cell division. Histone H3 is phosphorylated at residues Ser10 and Ser28, and is acetylated at Lys14. Phosphorylation at Ser10 occurs during entry into mitosis prior to chromatin condensation, and phosphorylation at Ser28 follows a similar pattern. In response to EGF stimulation, it has been proposed that sequential Ser10 phosphorylation, then Lys14 acetylation occurs, causing a change in chromatin structure and gene activation.
Product Details
More Information
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Applications Flow Cytometry, WB
Clone HisH3S10-4B6
Format Unconjugated
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser10 of human phospho histone H3
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Histone H3.1t, H3t, H3FT, HIST3H3
Uniprot ID Q16695
References Hans F and Dimitrov S. (2001) Oncogene. 20: 3021-3027.
Cheung P, Tanner KG, Cheung WL, et al. (2000) Molecular Cell. 5: 905-915.
Images
Cell Culture Products
Flow cytometric analysis of Hela cells secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green) using Phospho-Histone H3 (Ser10) antibody HisH3S10-4B6. Cat. #2061.
Cell Culture Products
Western blot analysis of Jurkat cell extract untreated or treated with calyculin A using Phospho-Histone H3 (Ser10) antibody HisH3S10-4B6. Cat. # 2061.
Cell Culture Products
Western blot analysis of A549 cell extract untreated or treated with nocodazole using 0.01 µg/mL Phospho-Histone H3 (Ser10) antibody HisH3S10-4B6 Cat. #2061 or Company C antibody at 0.03 µg/mL (manufacturer’s recommended concentration) developed using the same exposure.