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Phospho-Histone H3 (Ser28) (D6) rabbit mAb PE conjugate
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|Description||Histone H3 is one of the five main histone proteins involved in chromatin structure modification in eukaryotic cells. Histone proteins are highly post-translationally modified, including acetylation, phosphorylation, methylation and ubiquitination. Phospho histone H3 is the most extensively modified of the five histones. Phospho histone H3 is primarily acetylated at Lys9, 14, 18 and 23. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state.|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Formulation||1X PBS, 0.09% NaN3, 0.2% BSA|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Ser28 of human phospho Histone H3|
|Validated Reactivity||Human, Mouse|
|Recommended Usage||For flow cytometric staining, the suggested use of this reagent is 5µL per million cells or 5 µ per 100 µl of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
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Flow cytometric analysis of Hela cells stained and untreated (red) or stained and treated with nocodazole (green) using Phospho-Histone H3 (Ser28) PE conjugate antibody at 0.05 ug/mL HisH3S28-D6. Cat. #2047.
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