Phospho-Histone H3 (Ser28) (D6) rabbit mAb PE conjugate

Catalog
2047
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100 Tests(2047) $299.00
10 Tests(2047S) $99.00
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Product Overview
Description Histone H3 is one of the five main histone proteins involved in chromatin structure modification in eukaryotic cells. Histone proteins are highly post-translationally modified, including acetylation, phosphorylation, methylation and ubiquitination. Phospho histone H3 is the most extensively modified of the five histones. Phospho histone H3 is primarily acetylated at Lys9, 14, 18 and 23. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state.
Product Details
More Information
Applications Flow Cytometry
Clone HisH3S28-D6
Format PE
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser28 of human phospho Histone H3
Isotype Rabbit IgG1k
Preparation Protein A+G
Validated Reactivity Human, Mouse
Clonality Monoclonal
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5µL per million cells or 5 µ per 100 µl of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
References 1. Rosenfeld JA, et al. (2009) BMC Genomics. 10: 143.
2. Lachner M et al. (2001) Nature. 410: 116–20.
3. Hansen JC, et al. (1998) Biochemistry. 37: 17637-41.
4. Strahl BD and Allis, CD (2000) Nature. 403: 41-45.
5. Cheung P, et al. (2000) Cell. 103: 263-271.
6. Bernstein BE, and Schreiber SL (2002) Chem. Biol. 9, 1167-1173.
7. Thorne AW, et al. (1990) Eur. J. Biochem. 193: 701-713.
8. Hendzel MJ, et al. (1997) Chromosoma. 106: 348-360.
9. Goto H, et al. (1999) J. Biol. Chem. 274: 25543-25549.
10. Preuss U, et al. (2003) Nucleic Acids Res. 31: 878-885.
Storage 2-8ºC
Images
Cell Culture Products
Flow cytometric analysis of Hela cells stained and untreated (red) or stained and treated with nocodazole (green) using Phospho-Histone H3 (Ser28) PE conjugate antibody at 0.05 ug/mL HisH3S28-D6. Cat. #2047.