Phospho-Histone H3 (Ser28) (D6) rabbit mAb PE conjugate

Catalog
2047
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100 Tests(2047) $299.00
10 Tests(2047S) $99.00
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Product Overview
Description Histones are highly conserved proteins that serve the core of nucleosomes, which serve to organize chromatin fiber for DNA packing. Histone H3 phosphorylation plays a major role in both transcriptional activation, which requires unpacking of the chromatin structure, and in chromosome packing during cell division. Histone H3 is phosphorylated at residues Ser10 and Ser28, and is acetylated at Lys14. Phosphorylation at Ser10 occurs during entry into mitosis prior to chromatin condensation, and phosphorylation at Ser28 follows a similar pattern. In response to EGF stimulation, it has been proposed that sequential Ser10 phosphorylation, then Lys14 acetylation occurs, causing a change in chromatin structure and gene activation.
Product Details
More Information
Applications Flow Cytometry
Clone HisH3S28-D6
Format PE
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser28 of human phospho Histone H3
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage 2-8ºC
Pseudonyms Histone H3.1t, H3t, H3FT, HIST3H3
Uniprot ID Q16695
References Hans F and Dimitrov S. (2001) Oncogene. 20: 3021-3027.
Cheung P, Tanner KG, Cheung WL, et al. (2000) Molecular Cell. 5: 905-915.
Images
Cell Culture Products
Flow cytometric analysis of Hela cells stained and untreated (red) or stained and treated with nocodazole (green) using Phospho-Histone H3 (Ser28) PE conjugate antibody HisH3S28-D6. Cat. #2047.