Phospho-Btk (Tyr223) (B4) rabbit mAb

Catalog
1171
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200 uL (0.5 mg/mL)(1171) $475.00
20 uL (0.5 mg/mL)(1171S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description Btk is a major node in the B-cell receptor signaling pathway, where it regulates B cell maturation, activation, survival, differentiation, and proliferation. Btk is activated by Src family kinases, including Lyn, which phosphorylates Btk at Tyr551. Upon phosphorylation at this site, Btk is recruited to the plasma membrane where autophosphorylation at Tyr223 occurs. The Btk signaling pathway is a major target of small molecule inhibitors for B-cell lymphoma, autoimmune diseases, and non-Hodgkin’s lymphomas. These inhibitors either form a covalent bond at Cys481 in the ATP-binding site or serve as reversible inhibitors that bind the SH3 pocket and stabilize inactive Btk.
Product Details
More Information
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Applications Flow Cytometry
Clone BtkY223-B4
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human phospho Btk
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Bruton tyrosine kinase, Tyrosine-protein kinase BTK, Agammaglobulinemia tyrosine kinase, ATK, AGMX1, B-cell progenitor kinase, BPK
Uniprot ID Q06187
References Liang, C, Tian D, Ren X, et al. (2018) European Journal of Medicinal Chemistry. 151: 315-326.
Images
Cell Culture Products
Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa IL4 and PV (green) using 5 ng/mL of Phospho-Btk (Tyr223) antibody BtkY223-B4 Cat. #1171.
Cell Culture Products
Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (light blue) or untreated (red) or IFNα + IL4 + pervanadate-treated (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) Phospho-Btk (Tyr223) antibody BtkY223-B4 at 0.025µg/mL. Cat. #1171.
Cell Culture Products
Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or untreated (grey) or treated with IFNα + IL4 + pervanadate (orange) using 0.05 µg/mL isotype control Cat. #2141, or untreated (red) or treated (green) using Phospho-Btk (Tyr223) antibody BtkY223-B4 at 0.05µg/mL. Cat. #1171.