Phospho-Btk (Tyr223) (B4) rabbit mAb

Name: Phospho-Btk (Tyr223) (B4) rabbit mAb
SKU: 1171

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Catalog

1171

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Select Your Size	Price
200 uL (0.5 mg/mL)(1171)	$475.00
20 uL (0.5 mg/mL)(1171S)	$175.00

Size

$175.00

Qty

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Note

20 μL is enough antibody for at least 20 Western blots.

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Product Overview

Description	Btk is a major node in the B-cell receptor signaling pathway, where it regulates B cell maturation, activation, survival, differentiation, and proliferation. Btk is activated by Src family kinases, including Lyn, which phosphorylates Btk at Tyr551. Upon phosphorylation at this site, Btk is recruited to the plasma membrane where autophosphorylation at Tyr223 occurs. The Btk signaling pathway is a major target of small molecule inhibitors for B-cell lymphoma, autoimmune diseases, and non-Hodgkin’s lymphomas. These inhibitors either form a covalent bond at Cys481 in the ATP-binding site or serve as reversible inhibitors that bind the SH3 pocket and stabilize inactive Btk.

Product Details

More Information
Applications	Flow Cytometry
Clone	BtkY223-B4
Format	Unconjugated
Validated Reactivity	Human, Mouse
Cross Reactivity	Predicted to work with mouse, rat and other homologues.
Detection	Anti-Rabbit IgG
Clonality	Monoclonal
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human phospho Btk
Formulation	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype	Rabbit IgGk
Preparation	Protein A+G
Recommended Usage	1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage	-20ºC
Pseudonyms	Bruton tyrosine kinase, Tyrosine-protein kinase BTK, Agammaglobulinemia tyrosine kinase, ATK, AGMX1, B-cell progenitor kinase, BPK
Uniprot ID	Q06187
References	Liang, C, Tian D, Ren X, et al. (2018) European Journal of Medicinal Chemistry. 151: 315-326.

Images

Flow cytometric analysis of U937 cells untreated (Red) or treated with IFNa IL-4 and PV (green) using rabbit mAb Phospho-BTK (Tyr223) #1171, BTKY223-B4 at 0.01 ug/mL) or concentration-matched Rabbit (G9) mAb IgG Isotype Control #214 for cells untreated (black) or treated with IFNa, IL-4 and PV (blue).

Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (light blue) or untreated (red) or IFNα + IL4 + pervanadate-treated (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) Phospho-Btk (Tyr223) antibody BtkY223-B4 at 0.025µg/mL. Cat. #1171.

Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or untreated (grey) or treated with IFNα + IL4 + pervanadate (orange) using 0.05 µg/mL isotype control Cat. #2141, or untreated (red) or treated (green) using Phospho-Btk (Tyr223) antibody BtkY223-B4 at 0.05µg/mL. Cat. #1171.

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