Phospho-Btk (Tyr223) (B4) rabbit mAb

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Catalog

1171

Select Your Size	Price
200 uL (0.5 mg/mL)(1171)	$475.00
20 uL (0.5 mg/mL)(1171S)	$175.00

Size

$175.00

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Note

20 μL is enough antibody for at least 20 Western blots.

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Product Overview

Description	Bruton's tyrosine kinase (Btk) plays a major role in B cell development. B cell activation by various ligands is accompanied by Btk membrane translocation, where ransient phosphorylation at two sites, Tyr551 and Tyr223 create phospho Btk. Tyr551, in the activation loop, is transphosphorylated by the Src family of tyrosine kinases, leading to autophosphorylation at Tyr223. Tyr223 phosphorylation facilitates phospho Btk interaction with activated Syk. Defects in Btk are the cause of X-linked agammaglobulinemia (XLA); also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1).

Product Details

More Information
Applications	Flow Cytometry
Clone	BtkY223-B4
Format	Unconjugated
Cross Reactivity	Predicted to work with mouse, rat and other homologues.
Detection	Anti-Rabbit IgG
Formulation	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human phospho Btk
Isotype	Rabbit IgG1k
Preparation	Protein A+G
Validated Reactivity	Human, Mouse
Clonality	Monoclonal
Recommended Usage	1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
References	1. Khan WN, (2001) Immunol. Res. 23: 147-56. 2. Lewis CM, et al. (2001) Curr. Opin. Immunol. 13: 317-25. 3. Salim K, et al. (1996) EMBO J. 15: 6241-50. 4. Rameh LE, et al. (1997) J. Biol. Chem. 272: 22059-66. 5. Várnai P, et al. (1999) J. Biol. Chem. 274: 10983-9. 6. Rawlings, D.J. et al. (1996) Science 271: 822-5. 7. Park H, et al. (1996) Immunity 4: 515-25.
Storage	-20ºC

Images

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa IL4 and PV (green) using 5 ng/mL of Phospho-Btk (Tyr223) antibody BtkY223-B4 Cat. #1171.

Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (light blue) or untreated (red) or IFNα/IL4-treated (green) or untreated and blocked with phospho-peptide (black) or IFNα/IL4 and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or IFNα/IL4 and blocked with non-phospho peptide (purple) Phospho-Btk (Tyr223) antibody BtkY223-B4 at 0.05µg/mL. Cat. #1171.

BtkY223-B4 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or of Phospho-Btk (Tyr223) antibody BtkY223-B4 (green) Cat. #1171.

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