Sign up for the Abwiz Bio Newsletter to get actionable advice and exclusive deals direct to your inbox.
Phospho-Btk (Tyr223) (B4) rabbit mAb
20 μL is enough antibody for at least 20 Western blots.
Customers Also Viewed
|Description||Bruton's tyrosine kinase (Btk) plays a major role in B cell development. B cell activation by various ligands is accompanied by Btk membrane translocation, where ransient phosphorylation at two sites, Tyr551 and Tyr223 create phospho Btk. Tyr551, in the activation loop, is transphosphorylated by the Src family of tyrosine kinases, leading to autophosphorylation at Tyr223. Tyr223 phosphorylation facilitates phospho Btk interaction with activated Syk. Defects in Btk are the cause of X-linked agammaglobulinemia (XLA); also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1).|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human phospho Btk|
|Validated Reactivity||Human, Mouse|
|Recommended Usage||1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.|
|References||1. Khan WN, (2001) Immunol. Res. 23: 147-56.
2. Lewis CM, et al. (2001) Curr. Opin. Immunol. 13: 317-25.
3. Salim K, et al. (1996) EMBO J. 15: 6241-50.
4. Rameh LE, et al. (1997) J. Biol. Chem. 272: 22059-66.
5. Várnai P, et al. (1999) J. Biol. Chem. 274: 10983-9.
6. Rawlings, D.J. et al. (1996) Science 271: 822-5.
7. Park H, et al. (1996) Immunity 4: 515-25.
Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa IL4 and PV (green) using 5 ng/mL of Phospho-Btk (Tyr223) antibody BtkY223-B4 Cat. #1171.
Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (light blue) or untreated (red) or IFNα/IL4-treated (green) or untreated and blocked with phospho-peptide (black) or IFNα/IL4 and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or IFNα/IL4 and blocked with non-phospho peptide (purple) Phospho-Btk (Tyr223) antibody BtkY223-B4 at 0.05µg/mL. Cat. #1171.
BtkY223-B4 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or of Phospho-Btk (Tyr223) antibody BtkY223-B4 (green) Cat. #1171.
Phospho-PLCγ1 (Tyr783) (C4) rabbit mAbCatalog#: 2201
Phospho-c-Cbl (Tyr700) (E1) rabbit mAbCatalog#: 2321
Phospho-Stat4 (Tyr693) (F6) rabbit mAbCatalog#: 2281
Phospho-Stat4 (Tyr693) (F6) rabbit mAb PE conjugateCatalog#: 2282