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Phospho-Btk (Tyr223) (B4) rabbit mAb APC conjugate

Phospho-Btk (Tyr223) (B4) rabbit mAb APC conjugate

Name: Phospho-Btk (Tyr223) (B4) rabbit mAb APC conjugate
SKU: 1174

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Catalog

1174

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Select Your Size	Price
100 Tests(1174)	$299.00
10 Tests(1174S)	$99.00

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$99.00

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Product Overview

Description	Btk is a major node in the B-cell receptor signaling pathway, where it regulates B cell maturation, activation, survival, differentiation, and proliferation. Btk is activated by Src family kinases, including Lyn, which phosphorylates Btk at Tyr551. Upon phosphorylation at this site, Btk is recruited to the plasma membrane where autophosphorylation at Tyr223 occurs. The Btk signaling pathway is a major target of small molecule inhibitors for B-cell lymphoma, autoimmune diseases, and non-Hodgkin’s lymphomas. These inhibitors either form a covalent bond at Cys481 in the ATP-binding site or serve as reversible inhibitors that bind the SH3 pocket and stabilize inactive Btk.

Product Details

More Information
Applications	Flow Cytometry
Clone	BtkY223-B4
Format	APC
Validated Reactivity	Human, Mouse
Cross Reactivity	Predicted to work with mouse, rat and other homologues.
Detection	Anti-Rabbit IgG
Clonality	Monoclonal
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human phospho Btk
Formulation	1X PBS, 0.09% NaN3, 0.2% BSA
Isotype	Rabbit IgG1k
Preparation	Protein A+G
Recommended Usage	For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage	2-8ºC
Pseudonyms	Bruton tyrosine kinase, Tyrosine-protein kinase BTK, Agammaglobulinemia tyrosine kinase, ATK, AGMX1, B-cell progenitor kinase, BPK
Uniprot ID	Q06187
References	Liang, C, Tian D, Ren X, et al. (2018) European Journal of Medicinal Chemistry. 151: 315-326.

Images

Flow cytometric analysis of U937 cells unstained cells negative control (blue) or untreated (red) or treated with IFNa IL4 and PV (green) using Phospho-Btk (Tyr223) B4 APC conjugated antibody BtkY223-B4 Cat. #1174.

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