Phospho-Zap70 (Tyr319)/Syk (Tyr352) (A3) rabbit mAb SureLight 488 conjugate

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2080
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100 Tests(2080) $299.00
10 Tests(2080S) $99.00
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Product Overview
Description ZAP70 (Tyrosine-protein kinase ZAP-70, phospho Zap70) is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both phospho Zap70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. Phosphorylation of Tyr319 is required for the assembly of a phospho Zap70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells. The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1.
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Applications Flow Cytometry
Clone Zap70Y319-A3
Format SureLight 488
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr319/Tyr352 of human phospho Zap70/Syk.
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage 2-8ºC
Pseudonyms Tyrosine-protein kinase ZAP-70 , 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, SRK, Tyrosine-protein kinase SYK, Spleen tyrosine kinase, p72-Syk
Uniprot ID P43403
P43405
References 1. Brdicka T et al., (2005) Mol Cell Biol 25:4924–4933.
2. Chan AC et al., (1992) Cell 71:649–662.
3. Cheng AM et al., (1997) Proc Natl Acad Sci94:9797–9801.
4. Deindl S et al., (2007) Cell 129:735–746.
5. Elder ME et al., (1994) Science 264:1596–1599.
6. Negishi I et al., (1995) Nature 376:435–438.
7. Yokosuka T et al., (2005) Nat Immunol 6:1253–1262.
Images
Cell Culture Products
Flow cytometric analysis of Jurkat cells unstained untreated cells as negative control (blue) or stained untreated (red) or treated with pervanadate (green) using phospho-Zap70 (Tyr319)/Syk (Tyr352) antibody ZapY319-A3 Surelight® 488 conjugate Cat. #2080.