Phospho-Zap70 (Tyr319)/Syk (Tyr352) (A3) rabbit mAb PE conjugate

Catalog
2077
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100 Tests(2077) $299.00
10 Tests(2077S) $99.00
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Product Overview
Description

ZAP70 (Tyrosine-protein kinase ZAP-70, phospho Zap70) is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both phospho Zap70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. Phosphorylation of Tyr319 is required for the assembly of a phospho Zap70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells. The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1.

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Applications Flow Cytometry
Clone Zap70Y319-A3
Format PE
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr319/Tyr352 of human phospho Zap70/Syk.
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage 2-8ºC
Pseudonyms Tyrosine-protein kinase ZAP-70 , 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, SRK, Tyrosine-protein kinase SYK, Spleen tyrosine kinase, p72-Syk
Uniprot ID P43403
P43405
References 1. Brdicka T et al., (2005) Mol Cell Biol 25:4924–4933.
2. Chan AC et al., (1992) Cell 71:649–662.
3. Cheng AM et al., (1997) Proc Natl Acad Sci94:9797–9801.
4. Deindl S et al., (2007) Cell 129:735–746.
5. Elder ME et al., (1994) Science 264:1596–1599.
6. Negishi I et al., (1995) Nature 376:435–438.
7. Yokosuka T et al., (2005) Nat Immunol 6:1253–1262.
Images
Cell Culture Products
Flow cytometric analysis of Jurkat cells unstained untreated cells as negative control (blue) or stained and untreated (red) or stained and treated with pervanadate (green) using phospho-Zap70 (Tyr319)/Syk (Tyr352) antibody ZapY319-A3 PE conjugate Cat. #2077.