SARS-CoV-2 NP (84D7) rabbit mAb Biotin Conjugate

Catalog
2492
Select Your Size Price
200 uL (0.5 mg/mL)(2492) $800.00
20 uL (0.5 mg/mL)(2492S) $350.00
$350.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description

We have leveraged our next-generation rabbit mAb discovery platform to develop recombinant rabbit monoclonal antibodies with extremely high sensitivity and specificity to SARS-CoV-2. These antibodies, which include clones 75G5a (Abwiz Cat. #2481), 84C4a (Abwiz Cat. #2486), 84D7 (Abwiz Cat. #2491), and 85C1 (Abwiz Cat. #2496) can be paired in sandwich detection assay and used to detect nucleoprotein (NP) antigen from SARS-CoV-2. Antibodies 75G5a, 84C4a, and 85C1 do not cross-react to the highly related SARS-CoV virus or to any other coronaviruses tested. Sandwich ELISA detection using TMB/acid developer reliably detects NP antigen in the pg/mL range, and sensitivity is expected to be even higher when using more sensitive developer strategies. This panel of antibodies can be used as raw materials for diagnostic kits and can be applied to lateral flow systems for diagnostic detection of COVID-19.

Product Details
More Information
Applications ELISA
Clone 84D7
Format Biotin
Validated Reactivity Other
Cross Reactivity Highly-specific to SARS-CoV-2 nucleoprotein; does not cross-react with nucleoprotein from SARS-CoV or other coronaviruses.
Detection Streptavidin
Clonality Monoclonal
Immunogen SARS-CoV-2 nucleoprotein (NP) specific peptide
Formulation 1X PBS, 0.02% NaN3
Isotype Rabbit IgG1k
Preparation Protein A
Recommended Usage Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format.
Storage 2-8ºC
Pseudonyms Severe acute respiratory syndrome coronavirus 2 nucleoprotein, 2019-nCoV nucleocapsid protein
Uniprot ID P0DTC9
Images
Cell Culture Products
Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) at 1 ug/mL. Biotin-conjugated rabbit monoclonal antibody 84D7 (Cat# 2492) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to COVID-19 NP antigen.
Cell Culture Products
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 75G5a (Cat# 2481) as a capture antibody and 84D7-biotin (Cat# 2492) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 75G5a and 84D7-biotin detected COVID-19 NP antigen at very high sensitivity as low as 25 pg/mL (2.5 pg).