SARS-CoV-2 NP (84C4a) rabbit mAb

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200 uL (0.5 mg/mL)(2486) $800.00
20 uL (0.5 mg/mL)(2486S) $350.00

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20 μL is enough antibody for at least 20 Western blots. 

Product Overview

We have leveraged our next-generation rabbit mAb discovery platform to develop recombinant rabbit monoclonal antibodies with extremely high sensitivity and specificity to SARS-CoV-2. These antibodies, which include clones 75G5a (Abwiz Cat. #2481), 84C4a (Abwiz Cat. #2486), 84D7 (Abwiz Cat. #2491), and 85C1 (Abwiz Cat.#2496)  can be paired in sandwich detection assay and used to detect nucleoprotein (NP) antigen from SARS-CoV-2. Antibodies 75G5a, 84C4a, and 84D7 do not cross-react to the highly related SARS-CoV virus or to any other coronaviruses tested. Sandwich ELISA detection using TMB/acid developer reliably detects NP antigen in the pg/mL range, and sensitivity is expected to be even higher when using more sensitive developer strategies. This panel of antibodies can be used as raw materials for diagnostic kits and can be applied to lateral flow systems for diagnostic detection of COVID-19.

Product Details
More Information
Applications ELISA
Clone 84C4a
Format Unconjugated
Validated Reactivity Other
Cross Reactivity Highly-specific to SARS-CoV-2 nucleoprotein; does not cross-react with nucleoprotein from SARS-CoV or other coronaviruses.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen SARS-CoV-2 nucleoprotein (NP) specific peptide
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgG1k
Preparation Protein A
Recommended Usage Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format.
Storage -20ºC
Pseudonyms Severe acute respiratory syndrome coronavirus 2 nucleoprotein, 2019-nCoV nucleocapsid protein
Uniprot ID P0DTC9
Cell Culture Products
Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody 84C4a (Cat# 2486) was serially diluted 1:2 starting at 1 ug/mL, and shows very strong and specific binding to COVID-19 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.
Cell Culture Products
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 84C4a (Cat# 2486) as a capture antibody and 75G5a-biotin (Cat# 2482) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 84C4a and 75G5a-biotin detected COVID-19 NP antigen at very high sensitivity as low as 49 pg/mL (4.9 pg).