SARS-CoV-2 Nucleoprotein (NP) (75G5a) rabbit mAb Biotin Conjugate

Catalog #: 2482

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Select Your Size	Price
200 uL (0.5 mg/mL)(2482-200)	$570.00
20 uL (0.5 mg/mL)(2482-20)	$210.00

From $210.00

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20 μL is enough antibody for at least 20 Western blots.

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Product Overview

Description

We have leveraged our next-generation rabbit mAb discovery platform to develop recombinant rabbit monoclonal antibodies with extremely high sensitivity and specificity to SARS-CoV-2. These antibodies, which include clones 75G5a (Abwiz Cat. #2481), 84C4a (Abwiz Cat. #2486), 84D7 (Abwiz Cat. #2491), and 85C1 (Abwiz Cat. #2496) can be paired in sandwich detection assay and used to detect nucleoprotein (NP) antigen from SARS-CoV-2. Antibodies 75G5a, 84C4a, and 84D7 do not cross-react to the highly related SARS-CoV virus or to any other coronaviruses tested. Sandwich ELISA detection using TMB/acid developer reliably detects NP antigen in the pg/mL range, and sensitivity is expected to be even higher when using more sensitive developer strategies. This panel of antibodies can be used as raw materials for diagnostic kits and can be applied to lateral flow systems for diagnostic detection of COVID-19.

Product Details

Product Details
Specification	Description
Special Notice	This product is made to order. Please allow 1-2 business days for preparation and shipping.
Applications	ELISA
Clone	CoV2NP-75G5a
Format	Biotin
Validated Reactivity	Other
Cross Reactivity	Highly-specific to SARS-CoV-2 nucleoprotein; does not cross-react with nucleoprotein from SARS-CoV or other coronaviruses.
Detection	Streptavidin
Clonality	Monoclonal
Immunogen	SARS-CoV-2 nucleoprotein (NP) specific peptide
Formulation	1X PBS, 0.02% NaN3
Isotype	Rabbit IgGk
Preparation	Protein A
Recommended Usage	Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format.
Storage	2-8ºC
Pseudonyms	Severe acute respiratory syndrome coronavirus 2 nucleoprotein, 2019-nCoV nucleocapsid protein
Uniprot ID	P0DTC9

Images

Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) at 1 ug/mL. Biotin-conjugated rabbit monoclonal antibody 75G5a (Cat# 2482) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to COVID-19 NP antigen.

Western blot analysis of SARS-CoV-2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP nucleocapsid rabbit monoclonal antibody (75G5), CoV2NP-75G5a), Cat# 2481.

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 84C4a (Cat# 2486) as a capture antibody and 75G5a-biotin (Cat# 2482) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 84C4a and 75G5a-biotin detected COVID-19 NP antigen at very high sensitivity as low as 49 pg/mL (4.9 pg).

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 85C1 (Cat# 2496) as a capture antibody and 75G5a-biotin (Cat# 2482) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 85C1 and 75G5a-biotin detected COVID-19 NP antigen at very high sensitivity as low as 25 pg/mL (2.5 pg).

SARS-CoV-2 Nucleoprotein (NP) (75G5a) rabbit mAb Biotin Conjugate

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