Phospho-NFkB p65 (Ser529) (H3) rabbit mAb

Catalog
2406
Select Your Size Price
200 uL (0.5 mg/mL)(2406) $475.00
20 uL (0.5 mg/mL)(2406S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description

The nuclear factor κB (NFκB)/Rel family of transcription factors play a pivotal role in inflammatory and immune responses (1,2). NF-kappa-B is present in almost all cell types and is involved in many biological processes including immunity, inflammation, cell growth and differentiation, apoptosis, and tumorigenesis. NFkB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFkB1/p105, NFkB1/p50, REL and NFkB2/p52. The dimers bind at κB sites in the target gene DNA. Individual dimers have distinct preferences for different κB sites and can act as either transcriptional activators or repressors. NFkB Ser536 phosphorylation stimulates Lys310 acetylation and interaction of phospho NFkB with CBP. Acetylated/phospho NFkB induces enhanced transcriptional activity.

Product Details
More Information
Applications Flow Cytometry
Clone NFkBp65S529-H3
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser529 of human phospho-NFKB p65
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3
Uniprot ID Q04206
References 1. Baeuerle, P.A. and Henkel, T. (1994) Annu. Rev. Immunol. 12:141-179.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell. 87:13-20.
Images
Cell Culture Products
Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or untreated (red) or treated with TNFα + calyculin A (green) using Phospho-NFkB p65 (Ser529) antibody NFkBp65S529-H3 at 0.01µg/mL. Cat. #2406.
Cell Culture Products
Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or untreated (red) or treated with TNFα + calyculin A (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-NFkB p65 (Ser529) antibody NFkBp65S529-H3 at 0.01µg/mL. Cat. #2406.
Cell Culture Products
Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-NFkB p65 (Ser529) antibody NFkBp65S529-H3 at 1µg/mL. Cat. #2406.