Phospho-NFkB p65 (Ser536) (C8) rabbit mAb

Catalog
2311
Select Your Size Price
200 uL (0.5 mg/mL)(2311) $475.00
20 uL (0.5 mg/mL)(2311S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description The nuclear factor κB (NFκB)/Rel family of transcription factors play a pivotal role in inflammatory and immune responses (1,2). NF-kappa-B is present in almost all cell types and is involved in many biological processes including immunity, inflammation, cell growth and differentiation, apoptosis, and tumorigenesis. NFkB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFkB1/p105, NFkB1/p50, REL and NFkB2/p52. The dimers bind at κB sites in the target gene DNA. Individual dimers have distinct preferences for different κB sites and can act as either transcriptional activators or repressors. NFkB Ser536 phosphorylation stimulates Lys310 acetylation and interaction of phospho NFkB with CBP. Acetylated/phospho NFkB induces enhanced transcriptional activity.
Product Details
More Information
Applications WB
Clone NFkBS536-C8
Format Unconjugated
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser536 of human phospho NFkB.
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3
Uniprot ID Q04206
References 1. Baeuerle, P.A. and Henkel, T. (1994) Annu. Rev. Immunol. 12:141-179.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell. 87:13-20.
Images
Cell Culture Products
Western blot analysis of HeLa cell extract untreated or treated with TNFα + calyculin A using 0.01 µg/mL Phospho-NFkB (Ser536) antibody NFkBS536-C8. Cat.#2311.
Associated Products