Phospho-NFkB p65 (Ser529) (A2) rabbit mAb

Catalog #: 2401
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200 uL (0.5 mg/mL)(2401-200) $570.00
20 uL (0.5 mg/mL)(2401-20) $210.00
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20 μL is enough antibody for at least 20 Western blots.

Product Overview


The nuclear factor κB (NFκB)/Rel family of transcription factors play a pivotal role in inflammatory and immune responses (1,2). NF-kappa-B is present in almost all cell types and is involved in many biological processes including immunity, inflammation, cell growth and differentiation, apoptosis, and tumorigenesis. NFkB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFkB1/p105, NFkB1/p50, REL and NFkB2/p52. The dimers bind at κB sites in the target gene DNA. Individual dimers have distinct preferences for different κB sites and can act as either transcriptional activators or repressors. NFkB Ser536 phosphorylation stimulates Lys310 acetylation and interaction of phospho NFkB with CBP. Acetylated/phospho NFkB induces enhanced transcriptional activity.

Product Details

Product Details
Specification Description
Applications WB
Clone NFkBp65S529-A2
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser529 of human phospho-NFKB p65
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3
Uniprot ID Q04206
References 1. Baeuerle, P.A. and Henkel, T. (1994) Annu. Rev. Immunol. 12:141-179.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell. 87:13-20.


Cell Culture Products
Western blot analysis of NIH3T3 cell extract untreated or treated with TNFα and calyculin A using Phospho-NFkB p65 (Ser529) antibody NFkBS529-A2 at 0.05 µg/mL. Cat. #2401.
Cell Culture Products
Western blot analysis of NIH3T3 cell extract untreated or treated with TNFα + calyculin A using 0.05 µg/mL Phospho-NFkB p65 (Ser529) antibody NFkBS529-A2 or Company A antibody at 1:1000 dilution (manufacturer’s recommended concentration) developed using the same exposure.

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