Phospho-Ship2 (Tyr1135) (1D2) rabbit mAb

Catalog #: 2226
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200 uL (0.5 mg/mL)(2226) $475.00
20 uL (0.5 mg/mL)(2226S) $175.00
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Note

20 μL is enough antibody for at least 20 Western blots.

Product Overview

Description
Ship2 (SH2-containing inositol phosphatase 2, phospho Ship2) is a homolog of Ship1. Ship2 is highly expressed in the heart, in skeletal muscle, and in the placenta (1). SHIP2 negatively regulates insulin signaling (2), and Ship2 polymorphisms have been linked to hyperglycemia (3). Phospho Ship2 has been identified as a potential therapeutic target for the treatment of obesity and type 2 diabetes (4,5). The Ship2 residue Tyr1135 is phosphorylated in human cancer cells (6-8).

Product Details

Product Details
Specification Description
Applications Flow Cytometry
Clone Ship2Y1135-1D2
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat, and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr1135 of human phospho Ship2
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2, Inositol polyphosphate phosphatase-like protein 1, INPPL-1, Protein 51C, SH2 domain-containing inositol 5'-phosphatase 2
Uniprot ID O15357
References 1. Goss, V.L. et al. (2006) Blood 107, 4888-97.
2. Rikova, K. et al. (2007) Cell 131, 1190-203.
3. Pesesse, X. et al. (1997) Biochem Biophys Res Commun 239, 697-700.
4. Wada, T. et al. (2001) Mol Cell Biol 21, 1633-46.
5. Ishida, S. et al. (2006) Pancreas 33, 63-7.
6. Dyson, J.M. et al. (2005) Int J Biochem Cell Biol 37, 2260-5.
7. Liang, X. et al. (2006) Proteomics 6, 4554-64.
8. Guo, A. et al. (2008) Proc Natl Acad Sci U S A 105, 692-7.

Images

Cell Culture Products
Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNα IL-4 and pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Ship2 (Tyr1135) antibody Ship2Y1135-1D2 at 0.5µg/mL. Cat. #2226.
Cell Culture Products
Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 1 µg/mL of isotype control Cat. #2141 (orange) or untreated (red) or treated with staurosporine (green) using Phospho-Ship2 (Tyr1135) antibody Ship2Y1135-1D2 at 1 µg/mL. Cat. #2226.
Cell Culture Products
Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα IL-4 and pervanadate (green) using Phospho-Ship2 (Tyr1135) antibody Ship2Y1135-1D2 at 0.5µg/mL. Cat. #2226.

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