Phospho-S6 Ribosomal Protein (Ser235/236) (R3A2) rabbit mAb

Catalog
1191
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200 uL (0.5 mg/mL)(1191) $475.00
20 uL (0.5 mg/mL)(1191S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description

Mammalian ribosomal proteins comprise multigene families that consist predominantly of multiple processed pseudogenes and one functional intron-containing gene within their coding regions. Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein (40S ribosomal protein phospho S6, Phosphoprotein NP33, RPS6). Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions. S6 phosphorylation is stimulated by growth factors, tumor promoting agents and mitogens. It is dephosphorylated at growth arrest. Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the phospho S6 protein.

Product Details
More Information
Applications Flow Cytometry, WB
Clone S6S235S236-R3A2
Format Unconjugated
Cross Reactivity Predicted to work with mouse, rat, and other homologues.
Detection Anti-Rabbit IgG
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser235/236 of human phospho S6 Ribosomal Protein
Isotype Rabbit IgG1k
Preparation Protein A+G
Validated Reactivity Human, Mouse
Clonality Monoclonal
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
References 1. Magnuson et al. (2012) Biochemical Journal. 441 (1): 1–21.
2. Ruvinsky et al. (2006) Trends in Biological Sciences. 31 (6): 342–348.
Storage -20ºC
Images
Cell Culture Products
Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or treated with U0126 plus SB20350 (red) or treated with TPA plus calyculin A (green) using Phospho-S6 ribosomal protein (Ser235/Ser236) antibody S6S235S236-R3A2. Cat. #1191.
Cell Culture Products
Western blot analysis of NIH3T3 cell extract untreated or treated with 20% FBS using Phospho-S6 ribosomal protein (Ser235/Ser236) antibody S6S235S236-R3A2. Cat. #1191.
Cell Culture Products
Flow cytometric analysis of U937 cells, secondary antibody only negative control (blue) or treated with U0126 + SB20350 (red) or with TPA + calyculin A (green) using Phospho-S6 ribosomal protein (Ser235/Ser236) antibody S6S235S236-R3A2 (Abwiz Cat. #1191) or Company C antibody at 0.1ug/mL (manufacturer's recommended concentration).