Phospho-S6 Ribosomal Protein (Ser235/236) (R3A2) rabbit mAb PE conjugate

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1192
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100 Tests(1192) $299.00
10 Tests(1192S) $99.00
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Product Overview
Description Ribosomal protein S6 kinase is one of two parallel signaling pathways downstream of mTOR, with the other being 4E-BP1. mTOR phosphorylates and activates S6 kinase, which then phosphorylates ribosomal protein S6. The pathway regulates cell growth and cell cycle progression. The identified phosphorylation sites of S6 are Ser235, Ser236, Ser240, Ser244, and Ser247, which are evolutionarily conserved in higher eukaryotes. Ser236 has been proposed as the primary phosphorylation site. Studies using S6 knockin mice, where all five phosphorylation site serine residues are replaced by alanine, have provided extensive detail on S6 function. These studies support the role phosphorylated S6 plays in regulation of cell size, glucose homeostasis, and protein synthesis.
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Applications Flow Cytometry
Clone S6S235S236-R3A2
Format PE
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat, and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser235/236 of human phospho S6 Ribosomal Protein
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage 2-8ºC
Pseudonyms 40S ribosomal protein S6, Phosphoprotein NP33, Small ribosomal subunit protein eS6, RPS6
Uniprot ID P62753
References Ruvinsky I and Meyuhas O. (2006) TRENDS in Biochemical Sciences. 31: 342-348.
Images
Cell Culture Products
Flow cytometric analysis of U937 cells unstained U0126 plus SB20350 cells (blue) or stained and treated with U0126 plus SB20350 (red) or treated with TPA plus calyculin A (green) using phospho-S6 ribosomal protein (Ser235/Ser236) antibody S6S235S236-R3A2 PE conjugate. Cat. #1192.