Phospho-PLCγ1 (Tyr783) (C4) rabbit mAb

Catalog #: 2201
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200 uL (0.5 mg/mL)(2201) $475.00
20 uL (0.5 mg/mL)(2201S) $175.00
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Note

20 μL is enough antibody for at least 20 Western blots.

Product Overview

Description
The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositolphosphate to inositol triphosphate and diacylglycerol. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate diacylglycerols (DAGs) and water-soluble phosphorylated derivatives, such as inositol 1,4,5-triphosphate (IP3). Within the PLC family, PLCγ is the only member that contains SH2 and SH3 domains, necessary for phospho PLCγ activation. Phospho PLCγ, upon activation, can interact with receptor tyrosine kinases.

Product Details

Product Details
Specification Description
Applications Flow Cytometry
Clone PLCG1Y783-C4
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat, and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr783 of human phospho PLCγ1.
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, Phospholipase C-gamma-1, PLC-gamma-1, PLC1
Uniprot ID P19174
References 1. Singer, W.D. et al. (1997) Annu. Rev. Biochem. 66, 475–509.
2. Hernandez D, et al. (1994) Genomics 23 (2): 504–507.
3. Smrcka, A.V. et al. (1991) Science 251, 804–807.
4. Taylor, S.J. et al. (1991) Nature 350, 516–518.

Images

Cell Culture Products
Peptide blockage flow cytometric analysis of Hela cells secondary antibody only negative control (blue) treated with imatinib (red) treated with pervanadate (green) treated with PV + blocked with non-phospho- peptide (violet) or treated with PV + blocked with phospho-peptide (brown) using Phospho-PLCγ1 (Tyr783) antibody at 0.05 µg/mL PLCg1Y783-C4. Cat. #2201.
Cell Culture Products
PLCg1Y783-C4 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or of Phospho-PLCγ1 (Tyr783) antibody PLCg1Y783-C4 (green) Cat. #2201.
Cell Culture Products
Flow cytometric analysis of Hela cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.01 µg/mL Phospho-PLCγ1 (Tyr783) antibody PLCg1Y783-C4. Cat. #2201.
Cell Culture Products
Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-PLCγ1 (Tyr783) antibody PLCg1Y783-C4 (Abwiz Cat. #2201) or Company C antibody at 0.1 ug/mL (manufacturer's recommended concentration).

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