MSK1 (mitogen and stress activated protein kinase 1, phospho MSK1) is activated by Erk in response to growth factors and by and p38 in response to cellular stress (1). MSK1 is similar to RSK1 in that it has two kinase domains and a connecting regulatory linker region (2). S364/S381 phosphorylation activates RSK1 (3), which is analogous to residues S360 and S376 of MSK1, which may be important for phospho MSK1 activity.
Predicted to work with mouse, rat and other homologues.
Clonality
Monoclonal
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Thr581 of human phospho MSK1
Formulation
1X PBS, 0.09% NaN3, 0.2% BSA
Isotype
Rabbit IgGk
Preparation
Protein A+G
Recommended Usage
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage
2-8ºC
Pseudonyms
Ribosomal protein S6 kinase alpha-5, S6K-alpha-5, 90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen- and stress-activated protein kinase 1, RSK-like protein kinase, RSKL, RPS6KA5
1. Deak, M. et al. (1998) EMBO J. 17, 4426-4441.
2. Pierrat, B. et al. (1998) J. Biol. Chem. 273, 29661-29671.
3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
4. Markou, T. and Lazou, A. (2002) Biochem J 365, 757-63.
