Phospho-MCM2 (Ser139) (B12) rabbit mAb APC conjugate
The members of minichromosome maintenance (McM) protein family 2-7 were originally identified as a group of proteins essential for DNA replication (chromosomal maintenance (1,2). They share common sequence homology to each other in their nulceotide-binding domains and are distinct subgroup of the large AAA ATPase family, which are required for the initiation and elongation of DNA replication. It has been reported that Cdc7/Dbf4 phospohrylates MCM2 during G1/S cell cycle which coincides with the intiation of DNA replication (3,4)
|Validated Reactivity||Human, Mouse, Rat|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho MCM2|
|Formulation||1X PBS, 0.09% NaN3, 0.2% BSA|
|Recommended Usage||For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|Pseudonyms||DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, CCNL1, CDCL1, KIAA0030|
|References||1. Bell S.P. and Dutta A. (2002) Annu Rev Biochem71:333-74.
2. Chong J.P. et al., (2995) Natur 375: 418-21.
3. Donovan S. et al., 1997) Proc Natl Acad Sci USA, 94:5611-6.
4. Tsuji T et al., (2006) Mol Biol Cell17:4459-72.
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