Phospho-Zap70 (Tyr493)/Syk (Tyr526) (H11) rabbit mAb PE conjugate

Catalog #: 1162
Select Your Size Price
100 Tests(1162) $299.00
10 Tests(1162S) $99.00
From $99.00

Need to make a custom order?

Contact us


20 μL is enough antibody for at least 20 Western blots.

Product Overview


ZAP70 (Tyrosine-protein kinase ZAP-70, phospho Zap70) is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. Following TCR engagement, Zap70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both phospho Zap70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. Phosphorylation of Tyr319 is required for the assembly of a phospho Zap70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells. The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1.

Product Details

Product Details
Specification Description
Applications Flow Cytometry
Clone Zap70Y493-H11
Format PE
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat, and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr493 of human phospho Zap70
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage 2-8ºC
Pseudonyms Tyrosine-protein kinase ZAP-70 , 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, SRK, Tyrosine-protein kinase SYK, Spleen tyrosine kinase, p72-Syk
Uniprot ID P43403 P43405
References 1. Brdicka T, et al., (2005) Mol Cell Biol, 25:4924–4933.
2. Chan AC, et al., (1992) Cell, 71:649–662.
3. Cheng AM, et al., (1997) Proc Natl Acad Sci, 94:9797–9801.
4. Deindl S, et al., (2007) Cell, 129:735–746.
5. Elder ME, et al., (1994) Science, 264:1596–1599.
6. Negishi I, et al., (1995) Nature, 376:435–438.
7. Yokosuka T, et al., (2005) Nat Immunol, 6:1253–1262.


Cell Culture Products
Flow cytometric analysis of Jurkat cells unstained cells negative control (blue) or stained and untreated (red) or treated with pervanadate (green) using Phospho-Zap70 (Tyr493)/Syk (Tyr526)-PE Zap70Y493-H11-PE. Cat. #1162.

Associated Products