Phospho-Stat1 (Ser727) (C6) rabbit mAb APC conjugate

Catalog
2274
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100 Tests(2274) $299.00
10 Tests(2274S) $99.00
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Product Overview
Description Stat1 mediates the cellular response to IFNα, IFNβ, and IFNγ for the regulation of cell growth and the defense against viral and immune challenges. The Jak-Stat pathway plays a central role in the IFNγ response, where Stat1 phosphorylation on Tyr701 causes homodimerization through its SH2 domain, translocation to the nucleus, and binding to gamma-activated sequence (GAS) elements. Early in the activation sequence, Stat1 is also phosphorylated at Ser727 through a mechanism involving PI3 kinase and Akt. Stat1 has been found to correlate with increased resistance to chemotherapeutic drugs. However, Stat1 activation of the immune system helps suppress tumor growth, and multiple melanomas and squamous-cell carcinomas have been known to downregulate Stat1 expression to evade immune surveillance.
Product Details
More Information
Applications Flow Cytometry
Clone Stat1S727-C6
Format APC
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat, and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser727 of human phospho Stat1
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage 2-8ºC
Pseudonyms Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84
Uniprot ID P42224
References Ramana CV, Gil MP, Schreiber RD, and Stark GR. (2002) Trends in Immunology. 23: 96-101.
Avalle L, Pensa S, Regis G, Novelli F, and Poli V. (2012) JAK-STAT. 1: 65-72.
Images
Cell Culture Products
Flow cytometric analysis of U937 cells unstained untreated cells as negative control (blue) or stained untreated (red) or treated with IFNα IL-4 and pervanadate (green) using phospho-Stat1 (Ser727) antibody Stat1S727-C6 APC conjugate. Cat. #2274.