Phospho-Stat1 (Tyr701) (3E6) rabbit mAb

Catalog
2221
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200 uL (0.5 mg/mL)(2221) $475.00
20 uL (0.5 mg/mL)(2221S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description Stat1 mediates the cellular response to IFNα, IFNβ, and IFNγ for the regulation of cell growth and the defense against viral and immune challenges. The Jak-Stat pathway plays a central role in the IFNγ response, where Stat1 phosphorylation on Tyr701 causes homodimerization through its SH2 domain, translocation to the nucleus, and binding to gamma-activated sequence (GAS) elements. Early in the activation sequence, Stat1 is also phosphorylated at Ser727 through a mechanism involving PI3 kinase and Akt. Stat1 has been found to correlate with increased resistance to chemotherapeutic drugs. However, Stat1 activation of the immune system helps suppress tumor growth, and multiple melanomas and squamous-cell carcinomas have been known to downregulate Stat1 expression to evade immune surveillance.
Product Details
More Information
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Applications Flow Cytometry
Clone Stat1Y701-3E6
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat, and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr701 of human phospho Stat1
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84
Uniprot ID P42224
References Ramana CV, Gil MP, Schreiber RD, and Stark GR. (2002) Trends in Immunology. 23: 96-101.
Avalle L, Pensa S, Regis G, Novelli F, and Poli V. (2012) JAK-STAT. 1: 65-72.
Images
Cell Culture Products
Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα IL-4 and pervanadate (green) using Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 at 0.005 µg/mL Cat. #2221.
Cell Culture Products
Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNα IL-4 and pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 at 0.1µg/mL. Cat. #2221.
Cell Culture Products
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 at 0.1 µg/mL. Cat #2221.
Cell Culture Products
Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using 0.1 ug/mL of Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 Cat. #2221 or Company C antibody at 0.2 ug/mL (manufacturer's recommended concentration).