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Phospho-Stat1 (Tyr701) (3E6) rabbit mAb
20 μL is enough antibody for at least 20 Western blots.
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|Description||Stat1 is a signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), phospho Stat1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Phospho Stat1 becomes activated in response to KITLG/SCF and KIT signaling and may mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.|
|Cross Reactivity||Predicted to work with mouse, rat, and other homologues.|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Tyr701 of human phospho Stat1|
|Validated Reactivity||Human, Mouse|
|Recommended Usage||1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|References||1. Liu B, et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:10626-10631.
2. Ungureanu D, et al (2003) Blood 102:3311-3313.
3. Rogers R.S., et al. (2003) J. Biol. Chem. 278:30091-30097.
4. DeVries T.A., et al. (2004) J. Biol. Chem. 279:45603-45612.
5. Krejci P., et al. (2008) PLoS ONE 3:E3961-E3961.
Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα IL-4 and pervanadate (green) using Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 at 0.005 µg/mL Cat. #2221.
Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNα IL-4 and pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 at 0.1µg/mL. Cat. #2221.
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 at 0.1 µg/mL. Cat #2221.
Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using 0.1 ug/mL of Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 Cat. #2221 or Company C antibody at 0.2 ug/mL (manufacturer's recommended concentration).