Phospho-PLCγ2 (Tyr759) (G3) rabbit mAb APC conjugate
Catalog #: 1169
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The PLC-gamma isoforms of the PI-PLC family of lipases are regulated by growth factor receptors and B- and T-cell antigen receptors. While PLCγ1 is expressed ubiquitously, PLCγ2 is predominantly expressed in liver cells. PLCγ2 plays a dominant role in B-cell signaling. Btk directly phosphorylates PLCγ2, though the Syk kinase and BLNK adaptor protein are required. Both Tyr753 and Tyr759 have been identified as important phosphorylation sites for PLCγ2 activation in B-cells. PLCγ2 missense mutations and genomic deletions have been identified autoinflammatory diseases in humans. These include gain-of-function mutations, such as S707T, that possibly introduce an additional phosphorylation site and increase basal PLCγ2 activity.
|Validated Reactivity||Human, Mouse|
|Cross Reactivity||Predicted to work with mouse, rat, and other homologues.|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Tyr759 of human phospho PLCγ2.|
|Formulation||1X PBS, 0.09% NaN3, 0.2% BSA|
|Recommended Usage||For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.|
|Pseudonyms||1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2, Phosphoinositide phospholipase C-gamma-2, PLC-IV, PLC-gamma-2, PLCG2|
|References||Rodriguez R, Matsuda M, Perisic O, Bravo J, Paul A, Jones NP, Light Y, Swann K, Williams RL, and Katan M. (2001) Journal of Biological Chemistry. 276:47982-47992. Zhou Q, Lee G, Brady J et al. (2012) American Journal of Human Genetics. 4:713-720|
Flow cytometric analysis of Hela cells unstained imatinib treated cells (blue) or stained and treated with imatinib (red) or treated with pervanadate (green) using Phospho-PLCγ2 (Tyr759) antibody PLCG2Y759-G3 APC conjugate. Cat. #1169.