Phospho-PLCγ2 (Tyr759) (G3) rabbit mAb APC conjugate

Catalog #: 1169
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100 Tests(1169) $299.00
10 Tests(1169S) $99.00
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Note

20 μL is enough antibody for at least 20 Western blots.

Product Overview

Description
The PLC-gamma isoforms of the PI-PLC family of lipases are regulated by growth factor receptors and B- and T-cell antigen receptors. While PLCγ1 is expressed ubiquitously, PLCγ2 is predominantly expressed in liver cells. PLCγ2 plays a dominant role in B-cell signaling. Btk directly phosphorylates PLCγ2, though the Syk kinase and BLNK adaptor protein are required. Both Tyr753 and Tyr759 have been identified as important phosphorylation sites for PLCγ2 activation in B-cells. PLCγ2 missense mutations and genomic deletions have been identified autoinflammatory diseases in humans. These include gain-of-function mutations, such as S707T, that possibly introduce an additional phosphorylation site and increase basal PLCγ2 activity.

Product Details

Product Details
Specification Description
Applications Flow Cytometry
Clone PLCG2Y759-G3
Format APC
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat, and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr759 of human phospho PLCγ2.
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage 2-8ºC
Pseudonyms 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2, Phosphoinositide phospholipase C-gamma-2, PLC-IV, PLC-gamma-2, PLCG2
Uniprot ID P16885
References Rodriguez R, Matsuda M, Perisic O, Bravo J, Paul A, Jones NP, Light Y, Swann K, Williams RL, and Katan M. (2001) Journal of Biological Chemistry. 276:47982-47992.
Zhou Q, Lee G, Brady J et al. (2012) American Journal of Human Genetics. 4:713-720

Images

Cell Culture Products
Flow cytometric analysis of Hela cells unstained imatinib treated cells (blue) or stained and treated with imatinib (red) or treated with pervanadate (green) using Phospho-PLCγ2 (Tyr759) antibody PLCG2Y759-G3 APC conjugate. Cat. #1169.

Associated Products