Phospho-Histone H2A.X (Ser139) (1B3) rabbit mAb FITC conjugate

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Product Overview
Description Histone H2AX is a variant of the nucleosome core histone H2A and is phosphorylated at Ser139 in response to DNA damage. Histone H2AX phosphorylation is considered a specific reporter of double-strand DNA breaks. The protein is also referred to as γH2AX when phosphorylated at Ser139. H2AX phosphorylation is especially strong in response to double-strand breaks formed during apoptosis. However, physiological phosphorylation of Histone H2AX occurs when double-strand DNA breaks are formed during meiosis and V(D)J recombination. A549 and DU145 cell lines have been found to have higher expression levels of phosphorylated Histone H2AX compared to Jurkat, MCF-7, or HL-60 cell lines.
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Applications Flow Cytometry
Clone HisH2AXS139-1B3
Format FITC
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat, and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho histone H2A.X.
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage 2-8ºC
Pseudonyms H2AFX, H2AX, γH2AX, gamma-H2AX
Uniprot ID P16104
References Tanaka T, Halicka D, Huang X, et al. (2006) Cell Cycle. 5: 1940-1945.
Cell Culture Products
Flow cytometric analysis of 293T cells unstained cells (blue) or stained untreated (red) or treated with UV and TPA (green) using Phospho-Histone H2A.X (Ser139) antibody HisH2AXS139-1B3 FITC-conjugated. Cat. #2198.