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  1. Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and
    Human ACE2/ACEH Protein. Fc Tag
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  2. Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody 75G5a (Cat# 2481) was serially diluted 1:2 starting at 1 ug/mL, and shows very strong and specific binding to
    Recommended Pair - SARS-CoV-2 Nucleoprotein (NP) (75G5a) rabbit mAb
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  3. Recommended Pair - SARS-CoV-2 Nucleoprotein (NP) (85C10) rabbit mAb Biotin Conjugate
    Recommended Pair - SARS-CoV-2 Nucleoprotein (NP) (85C10) rabbit mAb Biotin Conjugate
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  4. The microtiter wells were coated with recombinant ACE2-Fc protein at 2 µg/mL. The spike trimer protein (96 ng/mL) was mixed with serially diluted antibody 1:1 at RT for 1 hour and added to the wells. Bound spike trimer was detected with horseradish peroxi
    SARS-CoV-2 6R8A-E11 Human Neutralizing mAb
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  5. Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with
    SARS-CoV-2 Alpha B.1.1.7 Variant Recombinant Spike Trimer His Tag
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  6. Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of
    SARS-CoV-2 B039 Omicron Variant Recombinant Spike Trimer His Tag
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  7. Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of
    SARS-CoV-2 BA.1 (B.1.1.529) Omicron Variant Recombinant Spike Trimer His Tag
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  8. Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of
    SARS-CoV-2 BA.2 Omicron Variant recombinant Spike Trimer His Tag
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  9. Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of
    SARS-CoV-2 BA.2.3.20 Omicron Variant Recombinant Spike Trimer His Tag
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  10. Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of
    SARS-CoV-2 BA.2.75 Omicron Variant Recombinant Spike Trimer His Tag
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  11. Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of
    SARS-CoV-2 BA.2.75.2 Omicron Variant Recombinant Spike Trimer His Tag
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  12. Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of
    SARS-CoV-2 BA.2.75.2+K444T Omicron Variant Recombinant Spike Trimer His Tag
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  13. Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of
    SARS-CoV-2 BA.2.75.2+V445P/F490S Omicron Variant Recombinant Spike Trimer His Tag
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  14. Concentration response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4C overnight. The wells were washed with PBS and blocked with 200 μL
    SARS-CoV-2 BA.2.86 Omicron Variant Recombinant Spike Trimer His Tag
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  15. Concentration response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4C overnight. The wells were washed with PBS and blocked with 200 μL
    SARS-CoV-2 BA.2.86.1 Omicron Variant Recombinant Spike Trimer His Tag
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  16. Concentration response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4C overnight. The wells were washed with PBS and blocked with 200 μL of
    SARS-CoV-2 BA.2.87.1 Omicron Variant Recombinant Spike Trimer His Tag
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  17. Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of
    SARS-CoV-2 BA.4.6 Omicron Variant Recombinant Spike Trimer His Tag
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  18. Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of
    SARS-CoV-2 BA.5 Omicron Variant Recombinant Spike Trimer His Tag
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  19. Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of
    SARS-CoV-2 BF.7/BA.5.2.6/BF.11 Omicron Variant Recombinant Spike Trimer His Tag
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  20. Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of ACE2-Fc at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of
    SARS-CoV-2 BN.1 Omicron Variant Recombinant Spike Trimer His Tag
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