Human ACE2/ACEH Protein. Fc Tag

Catalog #: 2566
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200 uL (0.5 mg/mL)(2566-200) $570.00
20 uL (0.5 mg/mL)(2566-20) $210.00
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Product Details

Product Details
Specification Description
Applications ELISA
Clone ACE2
Format Fc tag
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-SARS-CoV-2 mAb
Clonality SARS-CoV-2
Formulation 1X PBS, 0.09% NaN3
Isotype Other
Preparation Protein A
Recommended Usage For SARS-CoV-2 (COVID-19) diagnostic assays.
Storage 2-8ºC
Pseudonyms ACE2-2, ACEH, ACE2
Uniprot ID XXXXXX

Images

Cell Culture Products
Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).
Cell Culture Products
Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).
Cell Culture Products
Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).
Cell Culture Products
Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).
Cell Culture Products
Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).
Cell Culture Products
Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).
Cell Culture Products
Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).

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