Human ACE2/ACEH Protein. Fc Tag
Product Details
Specification | Description |
---|---|
Applications | ELISA |
Clone | ACE2 |
Format | Fc tag |
Validated Reactivity | Human |
Cross Reactivity | Predicted to work with mouse, rat and other homologues. |
Detection | Anti-SARS-CoV-2 mAb |
Clonality | SARS-CoV-2 |
Formulation | 1X PBS, 0.09% NaN3 |
Isotype | Other |
Preparation | Protein A |
Recommended Usage | For SARS-CoV-2 (COVID-19) diagnostic assays. |
Storage | 2-8ºC |
Pseudonyms | ACE2-2, ACEH, ACE2 |
Uniprot ID | XXXXXX |
Images

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).

Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).