Anti-PLXDC2 (4G3) rabbit mAb Biotin Conjugate

Catalog #: 2535
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200 uL (0.5 mg/mL)(2535-200 uL (0.5 mg/mL)) $475.00
20 uL (0.5 mg/mL)(2535-20 uL (0.5 mg/mL)) $175.00
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Short Description

Plexin domain containing protein 2 (PLXDC2), a cell surface transmembrane protein, is expressed in many tissues including haemotopoeitc stem cells, neural stem cells, pluripotent stem cells, and tumor cells. PLXDC2 may play an important role in neuronal growth, stem cell development, angiogenesis, and cancer cell growth. Recently PLXDC2 was reported a good cell surface marker as human haemotopoetic stem cells (1).

PLXDC2 has been reported as a receptor for pigment epithelium derived factor (PEDF) (2) or as an activating ligand for adhesion G-protein coupled receptor D1 (Adgrd1) (3). Also PLXDC2 was thought as a novel interaction partner and an entry receptor for rhesus monkey rhadinovirus (RRV) 4). The gene expression level of PLXDC2 was elevated in the peripheral blood of stroke patients (5) or in mouse bone marrow-derived macrophages in response to Helicobacter pylori (6). The protease BACE1 (β-Site APP Cleaving Enzyme), a major drug target in Alzheimer's disease, cleaves the amyloid precursor protein (APP) as well as PLXDC2 as one of several other substrates (7).

Citation for this Antibody

  1. Shimizu, H. et al. Improving the quality of a recombinant rabbit monoclonal antibody against PLXDC2 by optimizing transient expression conditions and purification method. Protein Expr. Purif. 146: 27–33 (2018).
  2. Yamamoto N. et al. Expression Pattern of Plexin Domain Containing 2 in Human Hepatocellular Carcinoma. Monoclon Antib Immunodiagn Immunother. 39(2):57-60 (2020)
  3. Hamada, Y. et al. Plexin domain containing protein 2 is more expressed within the invasive area of human colorectal cancer tissues. Human Cell 34(5): 1580-1583 (2021).
  4. Tanaka Y. et al. Prospective isolation of mouse and human hematopoetic stem cells using Plexin domain containing 2. bioRXiv Sep 27: 2021.09.27.461900v1.doi: 10.1101/2021.09.27.461900. (2021)
  5. Sakuma C. et al. Western blotting of native proteins from agarose gels Biotechniques Apr 6 (2022).
  6. Akuta T. et al. A new method to characterize conformation-specific antibody by a combination of agarose native gel electrophoresis and contact blotting. Antibodies 11(2): 36. (2022).

Product Details

Product Details
Specification Description
Applications WB
Clone 4G3
Format Biotin
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen HEK293 cells expressing human PLXDC2
Formulation 1X PBS, 0.05% (w/v) Ploclin300
Isotype Rabbit IgGk
Preparation Ion Exchange
Recommended Usage ELISA 1:5000-10000 Western blotting 1:1000-2000 ICH 1:1000-2000
Storage 2-8ºC
Pseudonyms TEM7R, 1200007L24Rik, 5430431D22Rik
Uniprot ID /Q6UX71
References 1. Tanaka Y et al 2021 bioRXiv Sep 27: 2021.09.27.461900v1.doi: 10.1101/2021.09.27.461900.
2. Cheng G et al. 2014 Elife. 3:e05401
3. Bianchi E et al 2021 Nat Commun. 2021 12:1251.
4. Großkopf AK et al. 2021 PLoS Pathog. 17:e1008979
5. O'Connell GC et al. 2017 Genom Data. 14:47-52.
6.) Tubau-Juni N, et al 2020 Sci. Rep. 10:11506.
7. Dislich B et al. 2015 Cell Proteomics. 10:2550-63.

Images

Cell Culture Products
Microtiter wells were coated with human PLXDC2-ECD (extracellular domain, truncated a transmembrane domain from PLXDC2 at 5 ug/mL. Anti-PLXDC2 (4G3) rabbit mAb Biotin Conjugate (Cat#2535) was diluted at 1 ug/mL and 0.1 ug/mL. Anti-PLXDC2 (4G3) rabbit mAb Biotin Conjugate show very specific binding to human PLXDC2-ECD antigen.
Cell Culture Products
A sandwich ELISA was performed using anti-PLXDC2 specific rabbit mAb: 3B8 (Cat#XXXX) as a capture antibody and 4G3-biotin (Cat#2535) as a detection antibody. Human PLXDC2-ECD was serially diluted 1:1 starting at 5 ug/mL. Anti-PLXDC2 rabbit mAb 3B8 and 4G3-biotin detected hPLXDC2-ECD antigen at very high sensitivity as low as 100 pg/mL.
Cell Culture Products
Western blot analysis of extracts from HEK293 cells, untransfected or transfected with human PLXDC2 gene, with anti-PLXDC2 (4G3) rabbit mAb Biotin Conjugate at 0.5 µg/mL Cat. #2535

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