CD22 is a transmembrane protein called Siglec-2 which is expressed as transmembrane protein in B-cells (1). It plays a critical role in regulating B cell function by inhibiting co-receptor for B cell receptor (BCR)(2). Phosphorylation of CD22 at Tyr822 induces recruiting SHP-1 tyrosine phosphatase which in turn dephosphorylates Syk, leading to a decrease in B cell activation pathways (3).
Predicted to work with mouse, rat and other homologues.
Detection
Anti-Rabbit IgG
Clonality
Monoclonal
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Tyr822 of human phospho CD22
Formulation
PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype
Rabbit IgGk
Preparation
Protein A
Recommended Usage
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
