We have leveraged our next-generation rabbit mAb discovery platform to develop recombinant rabbit monoclonal antibodies with extremely high sensitivity and specificity to SARS-CoV-2. These antibodies, which include clones 75G5a (Abwiz Cat. #2481), 84C4a (Abwiz Cat. #2486), 84D7 (Abwiz Cat. #2491), and 85C1 (Abwiz Cat. #2496) can be paired in sandwich detection assay and used to detect nucleoprotein (NP) antigen from SARS-CoV-2. Antibodies 75G5a, 84C4a, and 84D7 do not cross-react to the highly related SARS-CoV virus or to any other coronaviruses tested. Sandwich ELISA detection using TMB/acid developer reliably detects NP antigen in the pg/mL range, and sensitivity is expected to be even higher when using more sensitive developer strategies. This panel of antibodies can be used as raw materials for diagnostic kits and can be applied to lateral flow systems for diagnostic detection of COVID-19.
This product is made to order. Please allow 1-2 business days for preparation and shipping.
Applications
ELISA
Clone
Cov2NP-85C1
Format
Biotin
Validated Reactivity
Other
Cross Reactivity
Highly-sensitive to both SARS-CoV-2 and SARS-CoV nucleoprotein.
Detection
Streptavidin
Clonality
Monoclonal
Immunogen
SARS-CoV-2 nucleoprotein (NP) specific peptide
Formulation
1X PBS, 0.02% NaN3
Isotype
Rabbit IgGk
Preparation
Protein A
Recommended Usage
Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format.
Storage
2-8ºC
Pseudonyms
Severe acute respiratory syndrome coronavirus 2 nucleoprotein, 2019-nCoV nucleocapsid protein
