Cleaved PARP (Asp214) (H8) rabbit mAb APC conjugate

Catalog
2334
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100 Tests(2334) $299.00
10 Tests(2334S) $99.00
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Product Overview
Description Poly-ADP-ribose polymerase 1 (PARP-1), is a substrate of caspase-3 and caspase-7, both of which play a dominant role in apoptosis. PARP is cleaved into 89 and 24 kDa fragments at Asp214. The detection of these fragments is used as an indicator of caspase activation and apoptosis induction for many cell lines. Under normal conditions, PARP aids in the detection and repair of DNA damage. With 1-2 million copies per nucleus, PARP is also involved in poly (ADP-ribosyl)ation, a post-translational protein modification mechanism used to modify chromatin structure and regulate transcription. Decreased PARP activity has been shown to lead to loss of memory and neuronal cell death.
Product Details
More Information
Applications Flow Cytometry
Clone PARP-H8
Format APC
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic peptide corresponding to residues surrounding Asp214 of human PARP
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage 2-8ºC
Pseudonyms Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1
Uniprot ID P09874
References Bressenot A, Marchal S, Bezdetnaya L, Garrier J, Guillemin F, and Plenat F. (2009) Journal of Histochemistry and Cytochemistry. 57: 289-300.
Chaitanya GV, Alexander JS, and Babu, PP. (2010) Cell Communication and Signaling. 8:31.
Kraus WL, and Lis JT. (2003) Cell. 113:677-683.
Images
Cell Culture Products
Flow cytometric analysis of SK.N.MC cells unstained and untreated as negative control (blue) or stained untreated (red) or treated with staurosporine (green) using Cleaved PARP (Asp214) antibody PARP-H8 APC conjugate. Cat. #2334.