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Cleaved PARP (Asp214) (H8) rabbit mAb APC conjugate

Cleaved PARP (Asp214) (H8) rabbit mAb APC conjugate

Name: Cleaved PARP (Asp214) (H8) rabbit mAb APC conjugate
SKU: 2334

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Catalog

2334

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Select Your Size	Price
100 Tests(2334)	$299.00
10 Tests(2334S)	$99.00

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$99.00

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Product Overview

Description	Poly-ADP-ribose polymerase 1 (PARP-1), is a substrate of caspase-3 and caspase-7, both of which play a dominant role in apoptosis. PARP is cleaved into 89 and 24 kDa fragments at Asp214. The detection of these fragments is used as an indicator of caspase activation and apoptosis induction for many cell lines. Under normal conditions, PARP aids in the detection and repair of DNA damage. With 1-2 million copies per nucleus, PARP is also involved in poly (ADP-ribosyl)ation, a post-translational protein modification mechanism used to modify chromatin structure and regulate transcription. Decreased PARP activity has been shown to lead to loss of memory and neuronal cell death.

Product Details

More Information
Applications	Flow Cytometry
Clone	PARP-H8
Format	APC
Validated Reactivity	Human
Cross Reactivity	Predicted to work with mouse, rat and other homologues.
Detection	Anti-Rabbit IgG
Clonality	Monoclonal
Immunogen	A synthetic peptide corresponding to residues surrounding Asp214 of human PARP
Formulation	1X PBS, 0.09% NaN3, 0.2% BSA
Isotype	Rabbit IgG1k
Preparation	Protein A+G
Recommended Usage	For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage	2-8ºC
Pseudonyms	Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1
Uniprot ID	P09874
References	Bressenot A, Marchal S, Bezdetnaya L, Garrier J, Guillemin F, and Plenat F. (2009) Journal of Histochemistry and Cytochemistry. 57: 289-300. Chaitanya GV, Alexander JS, and Babu, PP. (2010) Cell Communication and Signaling. 8:31. Kraus WL, and Lis JT. (2003) Cell. 113:677-683.

Images

Flow cytometric analysis of SK.N.MC cells unstained and untreated as negative control (blue) or stained untreated (red) or treated with staurosporine (green) using Cleaved PARP (Asp214) antibody PARP-H8 APC conjugate. Cat. #2334.

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