Cleaved PARP (Asp214) (H8) rabbit mAb
Poly-ADP-ribose polymerase 1 (PARP-1), is a substrate of caspase-3 and caspase-7, both of which play a dominant role in apoptosis. PARP is cleaved into 89 and 24 kDa fragments at Asp214 (1). The detection of these fragments is used as an indicator of caspase activation and apoptosis induction for many cell lines. Under normal conditions, PARP aids in the detection and repair of DNA damage (2). With 1-2 million copies per nucleus, PARP is also involved in poly (ADP-ribosyl)ation, a post-translational protein modification mechanism used to modify chromatin structure and regulate transcription. Decreased PARP activity has been shown to lead to loss of memory and neuronal cell death (3).
|Applications||Flow Cytometry, WB|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Immunogen||A synthetic peptide corresponding to residues surrounding Asp214 of human PARP|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Recommended Usage||1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|Pseudonyms||Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1|
|References||1. Bressenot A, et al., (2009) J Histochem Cytochem. 57: 289-300.
2. Chaitanya GV, Alexander JS, and Babu, PP. (2010) Cell Comm Signal. 8:31.
3. Kraus WL, and Lis JT. (2003) Cell. 113:677-683.