TEC family of kinases (TEKs) represents a class of protein tyrosine kinases family that are implicated in Fc (episolon) RI-mediated cell activation (1). They are express in a wide range of cells, including hematopoietic cells including myeloid, B-, and T-cells lineages. They are one of the largest family of nonreceptor protein tyrosine kinases and consists of five members (2) : bone marrow kinase on the X chromosome (Bmx, also known as ETK) (3), Btk (4), interleukin (IL-2) inducible T-cell kinase (Itk; also known as Emt)((5), resting lymphocyte kinase Rtk, also known as Txk(6) and tyrosine kinase expressed in hepatocellular carcinoma (Tec)(7). Tec kinase is involved with LTC4 generation in mast cells. LT4 is generated from arachidonic acid through 5-lipoxygenase enzyme activity . Tec also is involved with cytokine production. In the absence of Tec, TNF alpha, IL-13 and GM-CSF levels are reduced, In addition, in the absence of Tec, production of IL-4 is completely abolished.
Predicted to work with mouse, rat and other homologues.
Detection
Anti-Rabbit IgG
Clonality
Monoclonal
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Tyr519 of human phospho Tec
Formulation
1X PBS, 0.09% NaN3, 0.2% BSA
Isotype
Rabbit IgGk
Preparation
Protein A
Recommended Usage
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
1. Felices M et al., 2007, Adv Immunol 93, 145-184. 2. Ortutary C et al., 2008, Adv Genet 64: 51-80. 3. Yamada N et al., 1993, Biochem Biophys Res Commun 192: 231-240. 4. Tsukada S et al., 1993, Cell 72:279-290. 5. Yamada N et al., 1993, Biochem Biophys Res Commun 192: 231-240. 6. Haire RN et al., 1994, Hum Mol Genet 3:897-901. 7. Mano H et al., 1990, Oncogene 5:1781-1786.
