Phospho-PTEN (Ser380/Thr382/383) (E4) rabbit mAb SureLight488 conjugate

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2135
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100 Tests(2135) $299.00
10 Tests(2135S) $99.00
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Product Overview
Description PTEN has been identified as a tumor suppressor gene and has been found to be mutated in a significant number of human cancers, including prostate, brain, and breast cancer. PTEN shares sequence homology with the protein-tyrosine phosphatase (PTPase) family of proteins and negatively regulates the PI3K/Akt pathway. PTEN de-phosphorylates target proteins, and recombinant PTEN has been shown to have phosphoinositide 3-phosphhatase and inositol phosphate 3-phosphatase activity. Studies of primary tumor cells show a loss of PTEN expression after metastasis to the brain, via astrocyte-derived microRNAs. A cluster of phosphorylation sites (S380, T382, T383, and S385) in the C-terminal tail of PTEN drive a conformational change that reduces PTEN activity by inhibiting membrane interactions.
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Applications Flow Cytometry
Clone PTENS380T382383-E4
Format SureLight 488
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser380 and Thr382/383 of human phospho PTEN
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage 2-8ºC
Pseudonyms Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase, Mutated in multiple advanced cancers 1, MMAC1, Phosphatase and tensin homolog, TEP1
Uniprot ID P60484
References Li J, Yen C, Liaw D, et al. (1997) Science. 275:1943-1947.
Maehama T, and Dixon JE. (1998) Journal of Biological Chemistry. 273:13375-13378.
Zhang L, Zhang S, You J, et al. (2015) Nature. 527:100-104.
Chen Z, Dempsey DR, Thomas SN, Hayward D, Bolduc DM, and Cole PA. (2016) Journal of Biological Chemistry. 291:14160-14169.
Images
Cell Culture Products
Flow cytometric analysis of A431 cells, unstained and untreated as negative control (blue) or stained and untreated (green) or stained and treated with lambda phosphatase (red) using Phospho-PTEN (Ser380/Thr382/383) antibody PTENS380T382383-E4 SureLight488 conjugate Cat. #2135.