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Phospho-PTEN (Ser380/Thr382/383) (E4) rabbit mAb APC conjugate
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|Description||PTEN has been identified as a tumor suppressor gene and has been found to be mutated in a significant number of human cancers, including prostate, brain, and breast cancer. PTEN shares sequence homology with the protein-tyrosine phosphatase (PTPase) family of proteins and negatively regulates the PI3K/Akt pathway. PTEN de-phosphorylates target proteins, and recombinant PTEN has been shown to have phosphoinositide 3-phosphhatase and inositol phosphate 3-phosphatase activity. Studies of primary tumor cells show a loss of PTEN expression after metastasis to the brain, via astrocyte-derived microRNAs. A cluster of phosphorylation sites (S380, T382, T383, and S385) in the C-terminal tail of PTEN drive a conformational change that reduces PTEN activity by inhibiting membrane interactions.|
|Validated Reactivity||Human, Mouse|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Ser380 and Thr382/383 of human phospho PTEN|
|Formulation||1X PBS, 0.09% NaN3, 0.2% BSA|
|Recommended Usage||For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.|
|Pseudonyms||Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase, Mutated in multiple advanced cancers 1, MMAC1, Phosphatase and tensin homolog, TEP1|
|References||Li J, Yen C, Liaw D, et al. (1997) Science. 275:1943-1947.
Maehama T, and Dixon JE. (1998) Journal of Biological Chemistry. 273:13375-13378.
Zhang L, Zhang S, You J, et al. (2015) Nature. 527:100-104.
Chen Z, Dempsey DR, Thomas SN, Hayward D, Bolduc DM, and Cole PA. (2016) Journal of Biological Chemistry. 291:14160-14169.
Flow cytometric analysis of A431 cells, unstained and untreated cells as negative control (blue) or stained untreated (green) or stained and treated with lambda phosphatase (red) using Phospho-PTEN (Ser380/Thr382/383) antibody PTENS380T382383-E4 APC conjugate Cat. #2134.