Phospho-MCM2 (Ser139) (B12) rabbit mAb

Catalog #: 2431
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200 uL (0.5 mg/mL)(2431) $475.00
20 uL (0.5 mg/mL)(2431S) $175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview

Description

The members of minichromosome maintenance (McM) protein family 2-7 were originally identified as a group of proteins essential for DNA replication (chromosomal maintenance (1,2). They share common sequence homology to each other in their nulceotide-binding domains and are distinct subgroup of the large AAA ATPase family, which are required for the initiation and elongation of DNA replication. It has been reported that Cdc7/Dbf4 phospohrylates MCM2 during G1/S cell cycle which coincides with the intiation of DNA replication (3,4) 

Product Details

Product Details
Specification Description
Applications Flow Cytometry, WB
Clone MCM2S139-B12
Format Unconjugated
Validated Reactivity Human, Mouse, Rat
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho MCM2
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, CCNL1, CDCL1, KIAA0030
Uniprot ID P49736
References 1. Bell S.P. and Dutta A. (2002) Annu Rev Biochem71:333-74. 2. Chong J.P. et al., (2995) Natur 375: 418-21. 3. Donovan S. et al., 1997) Proc Natl Acad Sci USA, 94:5611-6. 4. Tsuji T et al., (2006) Mol Biol Cell17:4459-72.

Images

Cell Culture Products
Flow cytometric analysis of C6 cells, secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.01 µg/mL. Cat. #2431.
Cell Culture Products
Western blot analysis of L929 cell extract untreated or treated with 25uM etoposide using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.05 ug/mL. Cat. #2431.
Cell Culture Products
Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (light blue) or untreated (red) or treated with staurosporine (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.1 µg/mL. Cat. #2431.
Cell Culture Products
Western blot analysis of L929 cell extract untreated or treated with etoposide using 0.05 ug/mL Phospho-MCM2 (Ser139) antibody MCM2S139-B12. Cat.#2431 or Company C antibody at 0.07 ug/mL (manufacturer’s recommended concentration) developed using the same exposure.
Cell Culture Products
Flow cytometric analysis of A431 cells, secondary antibody only negative control (blue), or untreated (grey) or treated with staurosporine (orange) using 0.01 ug/mL isotype control Cat. #2141, or untreated (red) or treated (green) using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.01 µg/mL. Cat. #2431.

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