The members of minichromosome maintenance (McM) protein family 2-7 were originally identified as a group of proteins essential for DNA replication (chromosomal maintenance (1,2). They share common sequence homology to each other in their nulceotide-binding domains and are distinct subgroup of the large AAA ATPase family, which are required for the initiation and elongation of DNA replication. It has been reported that Cdc7/Dbf4 phospohrylates MCM2 during G1/S cell cycle which coincides with the intiation of DNA replication (3,4)
Predicted to work with mouse, rat and other homologues.
Detection
Anti-Rabbit IgG
Clonality
Monoclonal
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho MCM2
Formulation
1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype
Rabbit IgGk
Preparation
Protein A+G
Recommended Usage
1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage
-20ºC
Pseudonyms
DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, CCNL1, CDCL1, KIAA0030
1. Bell S.P. and Dutta A. (2002) Annu Rev Biochem71:333-74.
2. Chong J.P. et al., (2995) Natur 375: 418-21.
3. Donovan S. et al., 1997) Proc Natl Acad Sci USA, 94:5611-6.
4. Tsuji T et al., (2006) Mol Biol Cell17:4459-72.
