Phospho-Lck (Tyr505) (A3) rabbit mAb FITC conjugate

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2303
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100 Tests(2303) $299.00
10 Tests(2303S) $99.00
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Product Overview
Description

Lck is a member of the Src family of non-receptor tyrosine kinases and plays a major role in T cell activation. Lck activates many downstream signaling pathways including Akt/mTOR, SAPK/JNK, PLCγ1, and RAS/MAPK. Phosphorylation of Lck at Tyr394 in the catalytic domain at the ATP-binding site stabilizes the open and active form, while phosphorylation at Tyr505 in the C-terminal domain promotes the closed, inactive conformation. Multiple small-molecule drugs used to treat leukemia have been shown to target inhibition of Lck, including imatinib and dasatinib. Lck is thus a promising target for suppressing T-cell responses for the treatment of inflammatory diseases or after organ transplantation.

Product Details
More Information
Applications Flow Cytometry
Clone LckY505-A3
Format FITC
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr505 of human phospho Lck
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage 2-8ºC
Pseudonyms Tyrosine-protein kinase Lck, Leukocyte C-terminal Src kinase, LSK, Protein YT16, T cell-specific protein-tyrosine kinase
Uniprot ID P)6239
References Serafin V, Capuzzo G, Milani G, et al. (2017) Blood. 130: 2750-2761.
Lee KC, Ouwehand I, Giannini AL, et al. (2010) Leukemia. 24: 896-900.
Images
Cell Culture Products
Flow cytometric analysis of Daudi Human Burkitt′s lymphoma cells untreated and unstained as negative control (blue) or untreated (red) or treated with IFNa +IL4 +PV and stained (green) using Phospho-LCK (Y505) antibody LCKY505-A3 FITC conjugate. Cat. #2303