Phospho-Lamin A/C (Ser22) (CF12) rabbit mAb FITC conjugate

Catalog
2438
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100 Tests(2438) $299.00
10 Tests(2438S) $99.00
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Product Overview
Description

Lamins are nuclear membrane proteins that are involved with cell cycle control, chromatin organization, and DNA replication (1,2). Upon caspase 6 cleavage, lamin A/C serves as a molecular marker for capspase 6 activity. Lamin A/C is cleaved during cellular apoptosis into a large (41-50 kDa) as well as a small (28 kDa) fragment leading to nuclear dysfunction and cell death (3) Phosphorylation of lamin A/C at Ser32 by MAPK/CDK signals cell cycle progression and mitosis (4,5).

Product Details
More Information
Applications Flow Cytometry
Clone LaminACS22-CF12
Format FITC
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser22 of human phospho Lamin A/C
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage 2-8ºC
Pseudonyms Prelamin-A/C, 70 kDa lamin, Renal carcinoma antigen NY-REN-32, LMNA, LMN1
Uniprot ID P02545
References 1. Goldberg, M, et al., (1999) Crit Rev Eukaryot Gene Expr, 9:285-93.
2. Yabuki, M., et ql., (1999) Physiol Chem Phys Med NMR 31: 77-84
3. Rao, L et al., (1996) J Cell Biol 135: 1441:55.
4. Orth K, et al., (1996) J Biol Chem 271:16443-6
5. Nousiainen M, et al., (2006) Proc Natl Acad Sci USA 103:5391-6
Images
Cell Culture Products
Flow cytometric analysis of Hela cells untreated (red) or treated with nocadozole (green) using Phospho-Lamin A/C (Ser22) (CF12) Rabbit mAb (FITC Conjugate) LaminACS22-CF12 #2438, or concentration-matched Rabbit (G9) mAb IgG Isotype Control (FITC Conjugate) #2143 for cells untreated (black) or treated with nocodazole (blue).