Phospho-Lamin A/C (Ser22) (CF12) rabbit mAb FITC conjugate
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2438
Lamins are nuclear membrane proteins that are involved with cell cycle control, chromatin organization, and DNA replication (1,2). Upon caspase 6 cleavage, lamin A/C serves as a molecular marker for capspase 6 activity. Lamin A/C is cleaved during cellular apoptosis into a large (41-50 kDa) as well as a small (28 kDa) fragment leading to nuclear dysfunction and cell death (3) Phosphorylation of lamin A/C at Ser32 by MAPK/CDK signals cell cycle progression and mitosis (4,5).
| Applications | Flow Cytometry |
|---|---|
| Clone | LaminACS22-CF12 |
| Format | FITC |
| Validated Reactivity | Human, Mouse |
| Cross Reactivity | Predicted to work with mouse, rat and other homologues. |
| Clonality | Monoclonal |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser22 of human phospho Lamin A/C |
| Formulation | 1X PBS, 0.09% NaN3, 0.2% BSA |
| Isotype | Rabbit IgGk |
| Preparation | Protein A+G |
| Recommended Usage | For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
| Storage | 2-8ºC |
| Pseudonyms | Prelamin-A/C, 70 kDa lamin, Renal carcinoma antigen NY-REN-32, LMNA, LMN1 |
| Uniprot ID | P02545 |
| References | 1. Goldberg, M, et al., (1999) Crit Rev Eukaryot Gene Expr, 9:285-93. 2. Yabuki, M., et ql., (1999) Physiol Chem Phys Med NMR 31: 77-84 3. Rao, L et al., (1996) J Cell Biol 135: 1441:55. 4. Orth K, et al., (1996) J Biol Chem 271:16443-6 5. Nousiainen M, et al., (2006) Proc Natl Acad Sci USA 103:5391-6 |
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