Phospho-HS1 (Tyr397) (F12) rabbit mAb

Catalog
2396
Select Your Size Price
200 uL (0.5 mg/mL)(2396) $475.00
20 uL (0.5 mg/mL)(2396S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description

HS1 is expressed in lymphoid and hematopoietic cells, and is heavily post-translationally modified. HS1 deficient mouse models have demonstrated the protein's role in receptor-mediated apoptosis and proliferation. HS1 is phosphorylated at Tyr378 and Tyr397 by the kinase Syk, providing a high-affinity binding site for SH2 domains from the Src family. Following this interaction, HS1 is then phosphorylated at Tyr222 by c-Fgr, Lyn, and Fyn kinases. HS1 plays an important role in T cell signaling, where HS1 phosphorylation recruits and activates Vav1 at the immune synapse. As a homolog of the actin binding protein cortactin, HS1 has been shown to mediate neutrophil chemotaxis through phosphorylation of tyrosines 222, 378, and 397.

Product Details
More Information
Applications Flow Cytometry, WB
Clone HS1Y397-F12
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr397 of human phospho HS1
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1, LckBP1, p75, HCLS1
Uniprot ID P14317
References Brunati AM, Donella-Deana A, James P, Quadroni M, Contri A, Marin O, and Pinna LA. (1999) Journal of Biological Chemistry. 274:7557-7564.
Cavnar PJ, Mogen K, Berthier E, Beebe DJ, and Huttenlocher A. (2012) Journal of Biological Chemistry. 287: 25466-25477.
Images
Cell Culture Products
Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-HS1 (Tyr397) antibody HS1Y397-F12 at 0.01µg/mL. Cat. #2396.
Cell Culture Products
Western blot analysis of Ramos cell extract, untreated or treated with 300 nM Thapsigargin for 30 min using HS1 (Tyr397) antibody HS1Y397-F12 at 0.01 µg/mL. Cat. #2396.
Cell Culture Products
Peptide blocking flow cytometric analysis of Ramos cells secondary antibody only negative control (light blue) or untreated (red) or treated with pervandadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-HS1 (Tyr397) antibody HS1Y397-F12 at 0.01µg/mL. Cat. #2396.
Cell Culture Products
Western blot analysis of Ramos cell extract untreated or treated with 300 nM thapsigargin for 30 min using 10 ng/mL Phospho-HS1 (Tyr397) antibody HS1Y397-F12 at 0.01µg/mL. Cat. #2396 or Company C antibody at 3 ng/mL (manufacturer’s recommended concentration) developed using the same exposure.
Cell Culture Products
Flow cytometric analysis of mouse cells secondary antibody only negative control (blue) or control (red) or stimulated (green) using Phospho-HS1 (Tyr397) antibody HS1Y397-F12 at 0.01µg/mL. Cat. #2396.