Phospho-c-Fos (Ser32) (BA9) rabbit mAb

Catalog
2426
Select Your Size Price
200 uL (0.5 mg/mL)(2426) $475.00
20 uL (0.5 mg/mL)(2426S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description

c-FOS belongs to the Fos family of nuclear oncogenes which include Fos B, Fos-related antigen 1 (FRA1), Fos-related antigen 2 (FRA2) in addition to c-Fos(1). Activator Protein-1 (AP-1) is formed upon dimerization of Fos proteins with Jun protiens (c-Jun, Jun B, and JunD)(2,3). AP-1 is considered a transcription factor that binds to TRE/AP-1 elements and activates transcription. ERK5 is involved with c-Fos phosphorylation at Ser32 and Thr232 which increase c-Fos stability and its nuclear translocation. 

Product Details
More Information
Applications Flow Cytometry
Clone cFosS32-BA9
Format Unconjugated
Validated Reactivity Human, Mouse, Rat
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser32 of human phospho c-Fos
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, G0S7
Uniprot ID P01100
References 1. Dobrazanski, P., et al., (1991) Mol Cell Biol, 11:5470-8.
2. Tulchinsky E (2000) Histol Histopathol 15:921-8.
3. Kovary K. and Bravo R. (1992) Mol Cell Biol 12:5015-23.
Images
Cell Culture Products
Flow cytometric analysis of HeLa cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV+TPA (green) using Phospho-c-Fos (Ser32) antibody cFosS32-BA9 at 0.001 µg/mL. Cat. #2426.
Cell Culture Products
Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or untreated (red) or treated with UV + TPA (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-c-Fos (Ser32) antibody cFosS32-BA9 at 0.01µg/mL. Cat. #2426.
Cell Culture Products
Flow cytometric analysis of L929 cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV (green) using Phospho-c-Fos (Ser32) antibody cFosS32-BA9 at 0.001 µg/mL. Cat. #2426.
Cell Culture Products
Flow cytometric analysis of C6 cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV+TPA (green) using Phospho-c-Fos (Ser32) antibody cFosS32-BA9 at 0.001 µg/mL. Cat. #2426.