Phospho-c-Fos (Ser32) (BA9) rabbit mAb
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Product Overview
Description
c-FOS belongs to the Fos family of nuclear oncogenes which include Fos B, Fos-related antigen 1 (FRA1), Fos-related antigen 2 (FRA2) in addition to c-Fos(1). Activator Protein-1 (AP-1) is formed upon dimerization of Fos proteins with Jun protiens (c-Jun, Jun B, and JunD)(2,3). AP-1 is considered a transcription factor that binds to TRE/AP-1 elements and activates transcription. ERK5 is involved with c-Fos phosphorylation at Ser32 and Thr232 which increase c-Fos stability and its nuclear translocation.
Product Details
| Specification | Description |
|---|---|
| Applications | Flow Cytometry |
| Clone | cFosS32-BA9 |
| Format | Unconjugated |
| Validated Reactivity | Human, Mouse, Rat |
| Cross Reactivity | Predicted to work with mouse, rat and other homologues. |
| Detection | Anti-Rabbit IgG |
| Clonality | Monoclonal |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser32 of human phospho c-Fos |
| Formulation | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA |
| Isotype | Rabbit IgGk |
| Preparation | Protein A+G |
| Recommended Usage | 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
| Storage | -20ºC |
| Pseudonyms | Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, G0S7 |
| Uniprot ID | P01100 |
| References | 1. Dobrazanski, P., et al., (1991) Mol Cell Biol, 11:5470-8. 2. Tulchinsky E (2000) Histol Histopathol 15:921-8. 3. Kovary K. and Bravo R. (1992) Mol Cell Biol 12:5015-23. |
Images
Flow cytometric analysis of HeLa cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV+TPA (green) using Phospho-c-Fos (Ser32) antibody cFosS32-BA9 at 0.001 µg/mL. Cat. #2426.
Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or untreated (red) or treated with UV + TPA (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-c-Fos (Ser32) antibody cFosS32-BA9 at 0.01µg/mL. Cat. #2426.
Flow cytometric analysis of L929 cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV (green) using Phospho-c-Fos (Ser32) antibody cFosS32-BA9 at 0.001 µg/mL. Cat. #2426.
Flow cytometric analysis of C6 cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV+TPA (green) using Phospho-c-Fos (Ser32) antibody cFosS32-BA9 at 0.001 µg/mL. Cat. #2426.
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