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Phospho-Stat6 (Tyr641) (G12) rabbit mAb
20 μL is enough antibody for at least 20 Western blots.
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|Description||Stat6 is a human protein encoded by the STAT6 gene and is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Phospho Stat6 plays a central role in exerting IL-4 and IL-13-mediated biological responses. In addition, Stat6 is activated by IFNα in B cell lines, where phospho Stat6 forms transcriptionally active complexes with Stat2 and p48. Stat6 has been found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL-4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and IgG class switching.|
|Applications||Flow Cytometry, WB|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Tyr641 of human phospho Stat6|
|Validated Reactivity||Human, Mouse|
|Recommended Usage||1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.|
|References||1. Leek JP. et al., (1997) Cytogenet. Cell Genet. 79: 208-9.
2. Hou J. et al., (1994) Science. 265: 1701-6.
3. Nelms, K. et al., (1999) Ann. Rev. Immunol. 17: 701-738.
4. Malabarba, M.G. et al., (1996) Biochem. J. 319: 865-872.
5. Hou, J. et al., (1994) Science. 265: 1701-1706.
6. Quelle, F.W. et al., (1995) Mol. Cell. Biol. 15: 3336-3343.
7. Takeda, K. et al., (1996) Nature. 380: 627-630.
8. Gupta, S. et al., (1999) J. Immunol. 163: 3834-3841.
Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa and IL-4 (green) using 0.1 µg/mL Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12. Cat. #1146.
Western blot analysis of Daudi cell extract untreated or treated with IL-4 using 0.01µg/mL Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12. Cat. #1146.
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12 at 0.1 µg/mL. Cat #1146.
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