Phospho-Stat6 (Tyr641) (G12) rabbit mAb
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Product Overview
Description
The transcription factor Stat6 is a member of the signal transducers and activators of transcription (STAT) family of proteins. Stat6 is the only member of this family that is activated by interleukin-4 (IL-4), after which Stat6 is both tyrosine- and serine-phosphorylated by Jak kinases. The consensus Stat6 binding site TTCN4GAA is found in the promoters of many genes regulated by IL-4. In T lymphocytes, Stat6 is required for differentiation into Th2 cells in response to IL-4. Stat6 may play a role in solid tumorigenesis; a large immunohistochemistry study of Stat6 expression in over 2,000 tumor samples confirmed strong nuclear staining.
Product Details
| Specification | Description |
|---|---|
| Applications | Flow Cytometry, WB |
| Clone | Stat6Y641-G12 |
| Format | Unconjugated |
| Validated Reactivity | Human, Mouse |
| Cross Reactivity | Predicted to work with mouse, rat and other homologues. |
| Detection | Anti-Rabbit IgG |
| Clonality | Monoclonal |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr641 of human phospho Stat6 |
| Formulation | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA |
| Isotype | Rabbit IgGk |
| Preparation | Protein A+G |
| Recommended Usage | 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
| Storage | -20ºC |
| Pseudonyms | Signal transducer and activator of transcription 6, IL-4 Stat |
| Uniprot ID | P42226 |
| References | Kaplan MH, Schindler U, Smiley ST, and Grusby MJ. (1996) Immunity. 4:313-319. Demicco EG, Harms PW, Patel RM, et al. (2015) American Journal of Clinical Pathology. 143:672-682. Wick KR, and Berton MT. (2000) Molecular Immunology. 37:641-652. |
Images
Western blot analysis of Daudi cell extract untreated or treated with IL-4 using 0.01µg/mL Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12. Cat. #1146.
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12 at 0.1 µg/mL. Cat #1146.
Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa and IL-4 (green) using 0.1 µg/mL Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12. Cat. #1146.
Western blot analysis of Daudi cell extract untreated or treated with IL-4 using 0.01 µg/mL Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12 Cat. #1146 or Company C antibody at 0.05 µg/mL (manufacturer’s recommended concentration) developed using the same exposure.
Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with IL-4 (green) using Phospho-Stat6 (Y641) antibody Stat6Y641-G12 (Abwiz Cat. #1146) or Company C antibody at 0.5 µg/mL (manufacturer's recommended concentration).
Associated Products
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Phospho-Stat6 (Tyr641) (G12) rabbit mAb FITC conjugateCatalog #: 1148 -
Phospho-Stat6 (Tyr641) (G12) rabbit mAb SureLight 488 conjugateCatalog #: 1150 -
Phospho-Stat6 (Tyr641) (G12) rabbit mAb PE conjugateCatalog #: 1147 -
Phospho-Stat6 (Tyr641) (G12) rabbit mAb APC conjugateCatalog #: 1149