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Phospho-Stat6 (Tyr641) (G12) rabbit mAb
20 μL is enough antibody for at least 20 Western blots.
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|Description||The transcription factor Stat6 is a member of the signal transducers and activators of transcription (STAT) family of proteins. Stat6 is the only member of this family that is activated by interleukin-4 (IL-4), after which Stat6 is both tyrosine- and serine-phosphorylated by Jak kinases. The consensus Stat6 binding site TTCN4GAA is found in the promoters of many genes regulated by IL-4. In T lymphocytes, Stat6 is required for differentiation into Th2 cells in response to IL-4. Stat6 may play a role in solid tumorigenesis; a large immunohistochemistry study of Stat6 expression in over 2,000 tumor samples confirmed strong nuclear staining.|
|Applications||Flow Cytometry, WB|
|Validated Reactivity||Human, Mouse|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Tyr641 of human phospho Stat6|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Recommended Usage||1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|Pseudonyms||Signal transducer and activator of transcription 6, IL-4 Stat|
|References||Kaplan MH, Schindler U, Smiley ST, and Grusby MJ. (1996) Immunity. 4:313-319.
Demicco EG, Harms PW, Patel RM, et al. (2015) American Journal of Clinical Pathology. 143:672-682.
Wick KR, and Berton MT. (2000) Molecular Immunology. 37:641-652.
Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa and IL-4 (green) using 0.1 µg/mL Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12. Cat. #1146.
Western blot analysis of Daudi cell extract untreated or treated with IL-4 using 0.01µg/mL Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12. Cat. #1146.
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12 at 0.1 µg/mL. Cat #1146.
Western blot analysis of Daudi cell extract untreated or treated with IL-4 using 0.01 µg/mL Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12 Cat. #1146 or Company C antibody at 0.05 µg/mL (manufacturer’s recommended concentration) developed using the same exposure.
Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with IL-4 (green) using Phospho-Stat6 (Y641) antibody Stat6Y641-G12 (Abwiz Cat. #1146) or Company C antibody at 0.5 µg/mL (manufacturer's recommended concentration).
Phospho-Stat6 (Tyr641) (G12) rabbit mAb FITC conjugateCatalog#: 1148
Phospho-Stat6 (Tyr641) (G12) rabbit mAb PE conjugateCatalog#: 1147
Phospho-Stat6 (Tyr641) (G12) rabbit mAb APC conjugateCatalog#: 1149