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Phospho-Stat3 (Tyr705) (B12) rabbit mAb
20 μL is enough antibody for at least 20 Western blots.
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|Description||Stat3 is a key signaling molecule for many cytokines and growth-factor receptors and is required for murine fetal development. Stat3 is constitutively activated in several human tumors and may be involved in oncogenic and anti-apoptotic functions. Phospho Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding. Phospho Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure or maturation stage of the cells. Notably, Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain.|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Tyr705 of human phospho Stat3|
|Validated Reactivity||Human, Mouse|
|Recommended Usage||1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|References||1. Heim, M.H. (1999) J. Recept. Signal Transduct. Res. 19:75-120.
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3. Catlett-Falcone, R. et al. (1999) Immunity. 10:105-115.
4. Garcia, R. and Jove, R. (1998) J. Biomed. Sci. 5:79-85.
5. Bromberg, J.F. et al. (1999) Cell. 98:295-303.
6. Darnell Jr., J.E. et al. (1994) Science. 264:1415-1421.
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Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα IL-4 and pervanadate (green) using Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (5 ng/mL). Cat. #1121.
Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) untreated (red) treated with IFNα IL-4 and pervanadate (green) treated + blocked with phospho-peptide (brown) using Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (5 ng/mL) Cat. #1121.
Stat3Y705-B12 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or of Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (green) Cat. #1121.
Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-Stat3 (Y705) antibody Stat3Y705-B12 (Abwiz Cat. #1121) or Company C antibody at 0.62 µg/mL (manufacturer's recommended concentration).