Sign up for the Abwiz Bio Newsletter to get actionable advice and exclusive deals direct to your inbox.
Purified anti-Phospho-Stat3 (Tyr705) rabbit mAb
Customers Also Viewed
|Description||Stat3 is a key signaling molecule for many cytokines and growth-factor receptors and is required for murine fetal development. Stat3 is constitutively activated in several human tumors and may be involved in oncogenic and anti-apoptotic functions. Phospho Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding. Phospho Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure or maturation stage of the cells. Notably, Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain.|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Validated Reactivity||Mouse, Human|
|Recommended Usage||1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.|
|References||1. Heim, M.H. (1999) J. Recept. Signal Transduct. Res. 19:75-120.
2. Takeda, K. et al. (1997) Proc. Natl. Acad. Sci. USA. 94:3801-3804.
3. Catlett-Falcone, R. et al. (1999) Immunity. 10:105-115.
4. Garcia, R. and Jove, R. (1998) J. Biomed. Sci. 5:79-85.
5. Bromberg, J.F. et al. (1999) Cell. 98:295-303.
6. Darnell Jr., J.E. et al. (1994) Science. 264:1415-1421.
7. Ihle, J.N. (1995) Nature. 377:591-594.
8. Wen, Z. et al. (1995) Cell. 82:241-250.
9. Yokogami, K. et al. (2000) Curr. Biol. 10:47-50.
10. Biethahn, S. et al. (1999) Exp. Hematol. 27:885-894.
Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue) or untreated (red) or treated with IFNÎ±, IL-4 and pervanadate (green) using Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (5 ng/mL). Cat. #1121.
Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue), untreated (red), treated with IFNÎ±, IL-4 and pervanadate (green), treated + blocked with phospho-peptide (brown) using Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (5 ng/mL) Cat. #1121.
Stat3Y705-B12 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells, secondary antibody only (blue) or 0.1 Âµg/mL of isotype control Cat. #2141 (orange) or of Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (green) Cat. #1121.
Conjugated anti-Phospho-Btk (Tyr223) APC rabbit mAbCatalog#: 1174
Purified anti-Phospho-Stat5 (Tyr694) rabbit mAbCatalog#: 2251
Conjugated anti-phospho-PLCγ1 (Tyr783) APC rabbit mAbCatalog#: 2204
Purified anti-phospho-PKCa (Thr497) rabbit mAbCatalog#: 2336