Purified anti-Phospho-Stat3 (Tyr705) rabbit mAb

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1121
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200 uL (0.5 mg/mL)(1121) $475.00
20 uL (0.5 mg/mL)(1121S) $175.00
$175.00
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Product Overview
Description Stat3 is a key signaling molecule for many cytokines and growth-factor receptors and is required for murine fetal development. Stat3 is constitutively activated in several human tumors and may be involved in oncogenic and anti-apoptotic functions. Phospho Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding. Phospho Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure or maturation stage of the cells. Notably, Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain.
Product Details
More Information
Format Unconjugated
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgG1κ
Preparation Protein A+G
Validated Reactivity Mouse, Human
Clonality Monoclonal
Recommended Usage 1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
References 1. Heim, M.H. (1999) J. Recept. Signal Transduct. Res. 19:75-120.

2. Takeda, K. et al. (1997) Proc. Natl. Acad. Sci. USA. 94:3801-3804.

3. Catlett-Falcone, R. et al. (1999) Immunity. 10:105-115.

4. Garcia, R. and Jove, R. (1998) J. Biomed. Sci. 5:79-85.

5. Bromberg, J.F. et al. (1999) Cell. 98:295-303.

6. Darnell Jr., J.E. et al. (1994) Science. 264:1415-1421.

7. Ihle, J.N. (1995) Nature. 377:591-594.

8. Wen, Z. et al. (1995) Cell. 82:241-250.

9. Yokogami, K. et al. (2000) Curr. Biol. 10:47-50.

10. Biethahn, S. et al. (1999) Exp. Hematol. 27:885-894.
Storage -20ºC
Images
Cell Culture Products
Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue) or untreated (red) or treated with IFNα, IL-4 and pervanadate (green) using Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (5 ng/mL). Cat. #1121.
Cell Culture Products
Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue), untreated (red), treated with IFNα, IL-4 and pervanadate (green), treated + blocked with phospho-peptide (brown) using Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (5 ng/mL) Cat. #1121.
Cell Culture Products
Stat3Y705-B12 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells, secondary antibody only (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or of Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (green) Cat. #1121.
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