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Phospho-SLP-76 (Tyr128) (3F8) rabbit mAb
20 μL is enough antibody for at least 20 Western blots.
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|Description||SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and composed of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues that are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with phospho SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that phospho SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76-deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Tyr128 of human phospho SLP-76|
|Validated Reactivity||Human, Mouse|
|Recommended Usage||1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|References||1. Clements, J.L. (2003) Immunol Rev 191, 211-9.
2. Bubeck Wardenburg, J. et al. (1998) Immunity 9, 607-16.
3. Bunnell, S.C. et al. (2000) J Biol Chem 275, 2219-30.
Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using 10 ng/mL Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8. Cat. #2136.
Peptide blocking flow cytometric analysis of Ramos cells secondary antibody only negative control (light blue) or untreated (red) or treated with pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 at 0.01µg/mL. Cat. #2136.
SLP76Y128-3F8 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or of Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 (green) Cat. #2136.
Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (grey) or treated with pervanadate (orange) using 10 ng/mL of isotype control Cat. #2141 or untreated (red) or pervanadate (green) using 10 ng/mL of Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 Cat. #2136 or Company B antibody at 10 ng/mL.
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