Phospho-SLP-76 (Tyr128) (3F8) rabbit mAb
Product Overview
Description
SH2 Domain-Containing Leukocyte Protein Of 76 KDa (SLP-76) is an adaptor protein that plays a role in signal transduction in T cells. Studies using a SLP-76-deficient T cell line have demonstrated that SLP-76 is required for optimal phosphorylation and activation of both PLCγ1 and the Ras pathway. SLP-76 phosphorylation is mediated by Zap70 upon TCR stimulation. Within an N-terminal acidic region, SLP-76 possesses three tyrosines (Tyr113, 128, and 145), which are phosphorylated upon activation. The sterile α-motif (SAM) domain of SLP-76 drives formation of dimers and higher order oligomers. SLP-76 micro-clusters at the immunological synapse enhance signal transduction and T cell activation.
Product Details
Specification | Description |
---|---|
Applications | Flow Cytometry |
Clone | SLP76Y128-3F8 |
Format | Unconjugated |
Validated Reactivity | Human, Mouse |
Cross Reactivity | Predicted to work with mouse, rat and other homologues. |
Detection | Anti-Rabbit IgG |
Clonality | Monoclonal |
Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr128 of human phospho SLP-76 |
Formulation | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA |
Isotype | Rabbit IgGk |
Preparation | Protein A+G |
Recommended Usage | 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
Storage | -20ºC |
Pseudonyms | Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP76, LCP2 |
Uniprot ID | Q13094 |
References | Zhang MS, Tran PM, Wolff AJ, Tremblay MM, Fosdick MG, and Houtman JCD. (2018) Science Signaling. 11:eaam9095. Yablonski D, Kuhne MR, Kadlecek T, and Weiss A. (1998) Science. 281:413-416. Thaker YR, Recino A, Raab M, Jabeen A, Wallberg M, Fernandez N, and Rudd CE. (2017) Journal of Biological Chemistry. 292:6281-6290. |
Images

Peptide blocking flow cytometric analysis of Ramos cells secondary antibody only negative control (light blue) or untreated (red) or treated with pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 at 0.01µg/mL. Cat. #2136.

SLP76Y128-3F8 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or of Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 (green) Cat. #2136.

Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using 10 ng/mL Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8. Cat. #2136.

Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (grey) or treated with pervanadate (orange) using 10 ng/mL of isotype control Cat. #2141 or untreated (red) or pervanadate (green) using 10 ng/mL of Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 Cat. #2136 or Company B antibody at 10 ng/mL.