Phospho-SLP-76 (Tyr128) (3F8) rabbit mAb

Catalog
2136
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200 uL (0.5 mg/mL)(2136) $475.00
20 uL (0.5 mg/mL)(2136S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description SH2 Domain-Containing Leukocyte Protein Of 76 KDa (SLP-76) is an adaptor protein that plays a role in signal transduction in T cells. Studies using a SLP-76-deficient T cell line have demonstrated that SLP-76 is required for optimal phosphorylation and activation of both PLCγ1 and the Ras pathway. SLP-76 phosphorylation is mediated by Zap70 upon TCR stimulation. Within an N-terminal acidic region, SLP-76 possesses three tyrosines (Tyr113, 128, and 145), which are phosphorylated upon activation. The sterile α-motif (SAM) domain of SLP-76 drives formation of dimers and higher order oligomers. SLP-76 micro-clusters at the immunological synapse enhance signal transduction and T cell activation.
Product Details
More Information
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Applications Flow Cytometry
Clone SLP76Y128-3F8
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr128 of human phospho SLP-76
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP76, LCP2
Uniprot ID Q13094
References Zhang MS, Tran PM, Wolff AJ, Tremblay MM, Fosdick MG, and Houtman JCD. (2018) Science Signaling. 11:eaam9095.
Yablonski D, Kuhne MR, Kadlecek T, and Weiss A. (1998) Science. 281:413-416.
Thaker YR, Recino A, Raab M, Jabeen A, Wallberg M, Fernandez N, and Rudd CE. (2017) Journal of Biological Chemistry. 292:6281-6290.
Images
Cell Culture Products
Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using 10 ng/mL Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8. Cat. #2136.
Cell Culture Products
Peptide blocking flow cytometric analysis of Ramos cells secondary antibody only negative control (light blue) or untreated (red) or treated with pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 at 0.01µg/mL. Cat. #2136.
Cell Culture Products
SLP76Y128-3F8 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or of Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 (green) Cat. #2136.
Cell Culture Products
Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (grey) or treated with pervanadate (orange) using 10 ng/mL of isotype control Cat. #2141 or untreated (red) or pervanadate (green) using 10 ng/mL of Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 Cat. #2136 or Company B antibody at 10 ng/mL.