Phospho-SAPK/JNK (Thr183/Tyr185) (A11) rabbit mAb

Catalog #: 2356
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200 uL (0.5 mg/mL)(2356) $475.00
20 uL (0.5 mg/mL)(2356S) $175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview

Description
The SAPK/JNK pathway initiates apoptosis upon exposure to radiation, UV exposure, heat shock, oxidative stress, and other stressors. Upon exposure to environmental stress, the SAPK/JNK signaling pathway sequentially activates the proteins MEKK1, SEK1, SAPK, and c-Jun. Upstream activators of the SAPK/JNK cascade include ceramide, small GTP-binding proteins such as Rac1 and Cdc42Hs, Ask1, and caspases. MKK7 is also a major and direct SAPK/JNK activator in the TNFα or environmental stress signaling pathways, where its kinase activity directly phosphorylates SAPK/JNK. This relationship between MKK7 and SAPK appears to be evolutionarily conserved, as it is preserved in their Drosophila homologues, Hep and DJNK, respectively.

Product Details

Product Details
Specification Description
Applications Flow Cytometry
Clone SAPKT183Y185-A11
Format Unconjugated
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of human phospho SAPK/JNK
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Mitogen-activated protein kinase 8, MAPK8, Stress-activated protein kinase 1c, SAPK1c, c-Jun N-terminal kinase 1, JNK1, PRKM8
Uniprot ID P45983
References Verheij M, Bose R, Lin XH, et al. (1996) Nature. 380: 75-79. Verheij M, Ruiter GA, Zerp SF, et al. (1998) Radiotherapy and Oncology. 47: 225-232. Moriguchi T, Toyoshima F, Masuyama N, et al. (1997) The EMBO Journal 16: 7045-7053.

Images

Cell Culture Products
Peptide blocking flow cytometric analysis of 293T cells secondary antibody only negative control (light blue) or treated with K252a (red) or with UV+TPA (green) or K252a and blocked with phospho-peptide (black) or UV and blocked with phospho pepeptide (gold) or K252a and blocked with non-phospho peptide (blue) or UV and blocked with non-phospho peptide (purple) using Phospho-SAPK/JNK (Thr183/Tyr185) antibody SAPKT183Y185-A11 at 0.01 µg/mL. Cat. #2356.
Cell Culture Products
Flow cytometric analysis of 293T cells secondary antibody only negative control (blue) or treated with K252a (red) or with UV+TPA (green) using Phospho-SAPK/JNK (Thr183/Tyr185) antibody SAPKT183Y185-A11 at 0.01 µg/mL. Cat. #2356.

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