Phospho-RelB (Ser552) (A7) rabbit mAb

Catalog #: 2206
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200 uL (0.5 mg/mL)(2206) $475.00
20 uL (0.5 mg/mL)(2206S) $175.00
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Note

20 μL is enough antibody for at least 20 Western blots.

Product Overview

Description
RelB contains the Rel homology Domain (RHD) shared by all members of the NF-kB family (1). It is best known for its roles in lymphoid development, DC biology, and noncanonical signaling (2). RelB is a major contributor to chromatin biology, frequently functioning as a dual transcription factor that silences sets of genes by generating silent facultative heterochromatin and activates euchromatin of others (3). RelB is required to repress immediate-response proinflammatory genes during endotoxin tolerance (4). The N-terminal leucine zipper motif of RelB, a motif unique among the NF-κB family, may associate with more diverse DNA sequences than other NF-κB members (5). RelB binds to DNA but only after forming a heterodimer with NF-kB p50 or p52 (6).
Like all NF-kB members, RelB contains an ∼300-residue region known as the RHD. This region supports many of the NF-kB essential functions, such as DNA binding, dimerization, and nuclear localization (7). RelB, unlike other NF-kB members, has an N-terminal leucine zipper motif (1), a domain that can typically interact with many proteins (8). p100, the C-terminus of NF-kB2 acts as a RelB inhibitor (9), where it sequesters RelB in the cytosol to repress its activity. RelB is phosphorylated rapidly at threonine 84 and serine 552, causing cleavage at the N-terminus and its degradation by the proteasome (10). These phosphorylations are mediated by glycogen synthase kinase-3β, specific inhibition of which blocks phospho RelB phosphorylation and degradation (11). This degradation does not occur when phospho RelB is phosphorylated at serine 368, allowing its association with and stabilization by p100 (12).

Product Details

Product Details
Specification Description
Applications Flow Cytometry
Clone RelBS552-A7
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser552 of human phospho RelB
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms I-Rel
Uniprot ID Q01201
References 1. Ryseck R P et al., (1992), Cell. Biol. 12, 674–684.
2. Vallabhapurapu S., and Karin M. (2009), Annu. Rev. Immunol. 27, 693–733.
3. McCall C E and Yoza B K, (2007) Am. J. Respir. Crit. Care Med. 175, 763–767.
4. Yoza B K et al., (2006) J. Immunol. 177, 4080–4085.
5. Liu T F et al., (2011) J. Biol. Chem. 286, 9856–9864.
5. Moorthy AK et al., (2007) J. Mol. Biol. 373, 723–734.
6. Bours V et al., (1994) Oncogene 9, 1699–1702.
7. Baldwin A. S., Jr., (1996) Annu. Rev. Immunol. 14, 649–683.
8. Alber T. (1992), Curr. Opin. Genet. Dev. 2, 205–210.
9. Dobrzanski P et al., (1993), Mol. Cell. Biol. 13, 1572–1582.
10 Marienfeld R et al., (2001), Oncogene 20, 8142–8147.
11. Neumann M. Et al., (2011), Oncogene 30, 2485–2492.
12 Maier HJ et al., (2003), J. Biol. Chem. 278, 39242–39250.

Images

Cell Culture Products
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or untreated (red) or treated with UV + TPA (green) using 0.01 µg/mL Phospho-RelB (Ser552) antibody RelBS552-A7. Cat. #2206.
Cell Culture Products
Peptide blocking flow cytometric analysis of 3T3 cells isotype antibody-stained negative control (light blue) or untreated (red) or treated with UV and PMA (green) or untreated and blocked with phospho-peptide (black) or UV/PMA and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or UV/PMA and blocked with non-phospho peptide (purple) using Phospho-RelB (Ser552) antibody RelBS552-A7 0.01µg/mL. Cat. #2206.
Cell Culture Products
Flow cytometric analysis of HeLa cells untreated (red) or treated with UV+TPA (green) using 0.1 µg/mL Phospho-RelB (Ser552) antibody RelBS552-A7. Cat. #2206, or concentration-matched rabbit (G9) mAb IgG isotope control #2141 for cells untreated (black) or treated with UV+TPA (blue).

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