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Phospho-Rb (Ser807/811) (D9) rabbit mAb
20 μL is enough antibody for at least 20 Western blots.
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|Description||Retinoblastoma protein (Rb, phospho Rb) is a tumor suppressor protein that is inactivated in a numbr of diverse cancers. The antiproliferative activity of Rb is mediated by its ability to inhibit the transcription of genes that are required for cell cycle progression. Rb contains conserved sites that are phosphorylated by cyclin-dependent kinases (CDKs). CDK phosphorylation typically promotes protein-protien interations through creation of a phospho-epitope that becomes structured upon binding its target. However Rb phosphorylation distrupts interactions with its binding partners. When it is phosphorylated, phospho Rb is inactivated and allows execcisve cell growth that is seen in cancer cells. Sixteen potential sites for CDK-mediated phosphorylation exist in Rb and twelve of these sites have been shown to be phosphorylated in vivo.|
|Applications||Flow Cytometry, WB|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Ser807/Ser811 of human phospho Rb|
|Validated Reactivity||Human, Mouse|
|Recommended Usage||1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.|
|References||1. Burkhart DL et al., (2008) Nat Rev Cancer. 8:671–682.
2. Knudsen ES (2008) Nat Rev Cancer. 8:714–724.
3. Mittnacht S. (1998) Curr Opin Genet Dev. 8:21–27.
4. Weinberg RA. (1995) 81:323–330.
5. Henley SA et al., (2012) Cell Div., 7:10.
Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with calyculin A (green) using Phospho-Rb (Ser807/Ser811) antibody RbS807S811-D9. Cat. #2051.
Western blot analysis of NIH3T3 cell extract untreated or treated with 20% FBS using Phospho-Rb (Ser807/Ser811) antibody RbS807S811-D9 at 0.1 µg/mL. Cat. #2051.
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