Phospho-Rb (Ser807/811) (D9) rabbit mAb

Catalog
2051
Select Your Size Price
200 uL (0.5 mg/mL)(2051) $475.00
20 uL (0.5 mg/mL)(2051S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description Retinoblastoma protein (Rb, phospho Rb) is a tumor suppressor protein that is inactivated in a numbr of diverse cancers. The antiproliferative activity of Rb is mediated by its ability to inhibit the transcription of genes that are required for cell cycle progression. Rb contains conserved sites that are phosphorylated by cyclin-dependent kinases (CDKs). CDK phosphorylation typically promotes protein-protien interations through creation of a phospho-epitope that becomes structured upon binding its target. However Rb phosphorylation distrupts interactions with its binding partners. When it is phosphorylated, phospho Rb is inactivated and allows execcisve cell growth that is seen in cancer cells. Sixteen potential sites for CDK-mediated phosphorylation exist in Rb and twelve of these sites have been shown to be phosphorylated in vivo.
Product Details
More Information
Applications Flow Cytometry, WB
Clone RbS807S811-D9
Format Unconjugated
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser807/Ser811 of human phospho Rb
Isotype Rabbit IgG1k
Preparation Protein A+G
Validated Reactivity Human, Mouse
Clonality Monoclonal
Recommended Usage 1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
References 1. Burkhart DL et al., (2008) Nat Rev Cancer. 8:671–682.

2. Knudsen ES (2008) Nat Rev Cancer. 8:714–724.

3. Mittnacht S. (1998) Curr Opin Genet Dev. 8:21–27.

4. Weinberg RA. (1995) 81:323–330.

5. Henley SA et al., (2012) Cell Div., 7:10.
Storage -20ºC
Images
Cell Culture Products
Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with calyculin A (green) using Phospho-Rb (Ser807/Ser811) antibody RbS807S811-D9. Cat. #2051.
Cell Culture Products
Western blot analysis of NIH3T3 cell extract untreated or treated with 20% FBS using Phospho-Rb (Ser807/Ser811) antibody RbS807S811-D9 at 0.1 µg/mL. Cat. #2051.