Phospho-Rb (Ser807/811) (D9) rabbit mAb

Catalog #: 2051
Select Your Size Price
200 uL (0.5 mg/mL)(2051) $475.00
20 uL (0.5 mg/mL)(2051S) $175.00
From $175.00

Need to make a custom order?

Contact us

Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview

Description
Retinoblastoma protein (Rb, phospho Rb) is a tumor suppressor protein that is inactivated in a numbr of diverse cancers. The antiproliferative activity of Rb is mediated by its ability to inhibit the transcription of genes that are required for cell cycle progression. Rb contains conserved sites that are phosphorylated by cyclin-dependent kinases (CDKs). CDK phosphorylation typically promotes protein-protien interations through creation of a phospho-epitope that becomes structured upon binding its target. However Rb phosphorylation distrupts interactions with its binding partners. When it is phosphorylated, phospho Rb is inactivated and allows execcisve cell growth that is seen in cancer cells. Sixteen potential sites for CDK-mediated phosphorylation exist in Rb and twelve of these sites have been shown to be phosphorylated in vivo.

Product Details

Product Details
Specification Description
Applications Flow Cytometry, WB
Clone RbS807S811-D9
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser807/Ser811 of human phospho Rb
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage Use 1 ug/mL for flow cytometry and 0.1 ug/mL for western blotting.
Storage -20ºC
Pseudonyms Retinoblastoma-associated protein, p105-Rb, pRb, pp110
Uniprot ID P06400
References 1. Burkhart DL et al., (2008) Nat Rev Cancer. 8:671–682.
2. Knudsen ES (2008) Nat Rev Cancer. 8:714–724.
3. Mittnacht S. (1998) Curr Opin Genet Dev. 8:21–27.
4. Weinberg RA. (1995) 81:323–330.
5. Henley SA et al., (2012) Cell Div., 7:10.

Images

Cell Culture Products
Flow cytometric analysis of U937 cells untreated and unstained as negative control (blue) or untreated and stained (green) or treated with lambda phosphatase and stained (red) using Phospho-Rb (Ser807/811) antibody RBS807S811-D9. at 1 ug/mL. Cat. #2051.
Cell Culture Products
Western blot analysis of NIH3T3 cell extract untreated or treated with 20% FBS using Phospho-Rb (Ser807/Ser811) antibody RbS807S811-D9 at 0.1 µg/mL. Cat. #2051.
Cell Culture Products
Western blot analysis of 3T3 cell extract untreated or treated with 20% FBS using 0.01 µg/mL Phospho-Rb (Ser807/811) antibody RbS807S811-D9 Cat. #2051 or Company C antibody at 0.1 µg/mL (manufacturer’s recommended concentration) developed using the same exposure.

Associated Products