Phospho-PLK1 (Thr210) (C2) rabbit mAb

Catalog #: 2346
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200 uL (0.5 mg/mL)(2346) $475.00
20 uL (0.5 mg/mL)(2346S) $175.00
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Note

20 μL is enough antibody for at least 20 Western blots.

Product Overview

Description
PLK1 is activated by phosphorylation of Thr210 in its activation loop. When dephosphorylated, PLK1 auto-inhibition occurs when the Polo-box domain (PDB) binds (and thus inhibits) the kinase domain. PLK1 phosphorylation is directly linked to the cell cycle, as phosphorylation occurs during mitosis but not during interphase. PLK1 is required for successful progression through the mitotic phase. Aurora A phosphorylates PLK1, with the G2-induced Bora protein directly interacting with PLK1 to relieve auto-inhibition. Small-molecule PLK1 inhibitors have played an important role in elucidating PLK1’s activities in the cell and have demonstrated the potential of PLK1 as an anti-cancer target. PLK1 inhibition is an efficient cell killer.

Product Details

Product Details
Specification Description
Applications Flow Cytometry
Clone PLK1T210-C2
Format Unconjugated
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Thr210 of human phospho PLK1
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Serine/threonine-protein kinase PLK1, Polo-like kinase 1, Serine/threonine-protein kinase 13, STPK13
Uniprot ID P53350
References Petronczki M, Lenart P, and Peters J. (2008) Developmental Cell. 14:646-659.
Seki A, Coppinger JA, Jang C, Yates III JR, and Fang G. (2008) Science. 320:1655-1658.

Images

Cell Culture Products
Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or untreated (red) or treated with nocodazole (green) or untreated and blocked with phospho peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-PLK1 (Thr210) antibody PLK1T210-C2 at 0.01 µg/mL. Cat. #2346.
Cell Culture Products
Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green) using Phospho-PLK1 (Thr210) antibody PLK1T210-C2 at 0.01 µg/mL. Cat. #2346.

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